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ITS-PCR-RFLP method for distinguishing commercial cultivars of edible mushroom, Flammulina velutipes

, : ITS-PCR-RFLP method for distinguishing commercial cultivars of edible mushroom, Flammulina velutipes. Journal of Food Science 67(7): 2486-2490

Nucleotide sequence and polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) analysis of the ribosomal RNA gene (rDNA) regions containing the internal transcribed spacers (ITSs) and the 5.8S rRNA coding sequence was used to differentiate between 7 typical Flammulina strains (MH09201, MH09208, MH09210, MH09234, MH09286, MH09289 and MH09297). These nucleotide sequences revealed the presence of strain-specific deletions, insertions and substitutions.


DOI: 10.1111/j.1365-2621.2002.tb08763.x

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