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Identification and application of RAPD markers for anthracnose resistance in water yam (Dioscorea alata)


, : Identification and application of RAPD markers for anthracnose resistance in water yam (Dioscorea alata). Annals of Applied Biology 141(1): 61-66

Anthracnose, caused by Colletotrichum gloeosporioides, is the most severe foliar disease of water yam (Dioscorea alata) worldwide. The tetraploid breeding line, TDa 95/00328, is a source of dominant genetic resistance to the moderately virulent fast growing salmon (FGS) strain of C. gloeosporioides. Bulked segregant analysis was used to search for random amplified polymorphic DNA (RAPD) markers linked to anthracnose resistance in F1 progeny derived from a cross between TDa 95/00328 and the susceptible male parent, TDa 95-310. Two hundred and eighty decamer primers were screened using bulks obtained from pooled DNA of individuals comprising each extreme of the disease phenotype distribution. A single locus that contributes to anthracnose resistance in TDa 95/00328 was identified and tentatively named Dcg-1. We found two RAPD markers closely linked in coupling phase with Dcg-1, named OPI71700 and OPE6950, both of which were mapped on the same linkage group. OPI71700 appeared tightly linked to the Dcg-1 locus; it was present in all the 58 resistant F1 individuals and absent in all but one of the 13 susceptible genotypes (genetic distance of 2.3cM). OPE6950 was present in 56 of the 58 resistant progeny and only one susceptible F1 plant showed this marker (6.8cM). Both markers successfully identified Dcg-1 in resistant D. alata genotypes among 34 breeding lines, indicating their potential for use in marker-assisted selection. OPI71700 and OPE6950 are the first DNA markers for yam anthracnose resistance. The use of molecular markers presents a valuable strategy for selection and pyramiding of anthracnose resistance genes in yam improvement. Reprinted by permission of the publisher.

US$19.90

DOI: 10.1111/j.1744-7348.2002.tb00195.x


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Smirnova, O.V., 2012: Sex differences in drug action: the role of multidrug-resistance proteins (MRPs). The mechanisms of participation of multidrug-resistance proteins (MRPs) in sex difference pattern of drug efficiency and side effects have been analyzed. MRPs structure, their tissue and cellular localization, substrate specificity, and functions...

Thiaucourt, F.; Bölske, G., 1996: Contagious caprine pleuropneumonia and other pulmonary mycoplasmoses of sheep and goats. Contagious caprine pleuropneumonia (CCPP) is now a well-defined disease that is caused by Mycoplasma capricolum subsp. capripneumoniae. CCPP is infectious, contagious and fulfils the classic Koch postulates that characterise such types of disease....

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D.Clercq, F., 1953: Hodgkin's disease and sarcoidosis; radiological aspects of thorax in cardiac insufficiency. Bruxelles Medical 33(9): 450-463

Hansch, R.; Koprek, T.; Heydemann, H.; Mendel, R.R.; Schulze, J., 1996: Electroporation-mediated transient gene expression in isolated scutella of Hordeum vulgare. Electroporation was used to introduce DNA into excised scutella of immature embryos of Hordeum vulgare L. cvs Golden Promise and Delita. Using the firefly luciferase gene as reporter, parameters were analyzed for high transient gene expression whi...

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Omata, K.; Abe, K., 1990: Plasma and urinary kinin assay and the clinical significance. Nihon Rinsho. Japanese Journal of Clinical Medicine 48 Suppl: 117-121

Iwanami, A.; Kanamori, R.; Isono, H.; Okajima, Y.; Kamijima, K., 1996: Impairment of inhibition of unattended stimuli in schizophrenic patients: event-related potential correlates during selective attention. Auditory event-related potentials (ERPs) were recorded during a dichotic listening task in 18 remitted schizophrenic patients and in 18 age-matched controls. The subjects were instructed to press a button in response to the target tones applied to...

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