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Identification and biochemical characterization of a barley nuclease involved in microspore growth and development

, : Identification and biochemical characterization of a barley nuclease involved in microspore growth and development. Biotechnological approaches for utilisation of gametic cells COST 824: Final Meeting, Bled, Slovenia, 1-5 July 2000: 261-267

It has been previously demonstrated that barley (Hordeum vulgare) microspores at the mid-to-late uninucleate stage display strong nucleolytic activity. In this work, a nuclease was partially purified from diffusates of barley (cv. Arda) microspores by using concanavalin A as ligand. DNase in-gel assay showed that the chromatographic preparation contain a single 34-kDa protein with nucleolytic activity.


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