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Identification and characterization of Fugu orthologues of mammalian interleukin-12 subunits

, : Identification and characterization of Fugu orthologues of mammalian interleukin-12 subunits. Immunogenetics 55(5): 296-306

We have isolated and characterized cDNAs and genes for pufferfish, Fugu rubripes, (Fugu) orthologues of mammalian interleukin (IL)-12 subunits (IL-12 p35 and IL-12 p40). The deduced amino acid sequences of the Fugu IL-12 subunits showed homology with mammalian IL-12 subunits (p35: 50.4-58.0% similarity; p40: 51.2-55.4% similarity). Phylogenetic analysis confirmed that Fugu IL-12 p35 and p40 genes cluster with their mammalian counterpart lineages. The genomic organization of each of the Fugu IL-12 subunit genes is similar to that of the corresponding mouse IL-12 subunit genes, although the Fugu genes are very compact due to small intron size. Comparative genomic analysis showed conserved syntenies within the IL-12 p35 and p40 regions between Fugu and human, indicating that the Fugu IL-12 p35 and p40 genes are orthologues for mammalian IL-12 p35 and p40 encoding genes, respectively. Expression of IL-12 p35 mRNA was observed in lymphoid tissues and several non-lymphoid tissues, while expression of IL-12 p40 mRNA was constitutive and nearly ubiquitous. In the spleen and head kidney, expression of IL-12 p35 was induced by polyriboinosinic polyribocytidylic acid [poly(I:C)] and not by lipopolysaccharide (LPS), while expression of IL-12 p40 was constitutive and unresponsive to both poly(I:C) and LPS. These results indicate that IL-12 levels are regulated by production of IL-12 p35 mRNA and suggest that IL-12 in fish may be involved in antiviral defense. This is the first report of the identification and characterization of IL-12 subunit cDNAs and genes in a non-mammalian vertebrate.


PMID: 12845500

DOI: 10.1007/s00251-003-0582-9

Other references

Lee, S.J.; Gralla, J.D., 2001: Sigma38 (rpoS) RNA polymerase promoter engagement via -10 region nucleotides. Band shift assays using DNA probes that mimic closed and open complexes were used to explore the determinants of promoter recognition by sigma38 (rpoS) RNA polymerase. Duplex recognition was found to be much weaker than that observed in sigma70 pr...

Rose R.C., 1987: Solubility properties of reduced and oxidized ascorbate as determinants of membrane permeation. The oil/water distribution coefficients of ascorbic acid and dehydro-L-ascorbic acid have been determined and compared with values for mannitol and lauric acid. In general, the relative degrees of hydrophobicity of the compounds evaluated are laur...

Larkin, H., 1990: Regulation, fiscal pressure expand CEO planning role. In an environment of strict fiscal control and continuing pressure to control costs, many CEOs are becoming actively involved in health planning at the local level. Most experts agree that regulators aren't likely to bring back certificate of...

Nehez M.; Selypes A.; Paldy A.; Mazzag E.; Berencsi G.; Jarmay K., 1981: Histo pathological and cyto genetic picture of micro toxicological effects of 2 nitro phenol structured pesticides in mouse experiments on germ cells. Mutation Research 85(4): 256

Xiong, X.; Zhou, K-Ming.; Wu, Z-Xing.; Xu, T., 2006: Silence of synaptotagmin I in INS-1 cells inhibits fast exocytosis and fast endocytosis. Synaptotagmin I (Syt I) is a Ca2+ sensor for triggering fast synchronized release of neurotransmitters. However, controversy remains whether Syt I is also obligatory for the exocytosis and endocytosis of larger dense core vesicles (LDCVs) in endoc...

Braend M., 1970: 11th european conference on animal blood groups and biochemical polymorphism warsaw july 2nd 6th 1968. European Conference On Animal Blood Groups And Biochemical Polymorphism:

Kido-Nakahara, M.; Buddenkotte, Jörg.; Kempkes, C.; Ikoma, A.; Cevikbas, F.; Akiyama, T.; Nunes, F.; Seeliger, S.; Hasdemir, B.; Mess, C.; Buhl, T.; Sulk, M.; Müller, F-Ulrich.; Metze, D.; Bunnett, N.W.; Bhargava, A.; Carstens, E.; Furue, M.; Steinhoff, M., 2014: Neural peptidase endothelin-converting enzyme 1 regulates endothelin 1-induced pruritus. In humans, pruritus (itch) is a common but poorly understood symptom in numerous skin and systemic diseases. Endothelin 1 (ET-1) evokes histamine-independent pruritus in mammals through activation of its cognate G protein-coupled receptor endothel...

Brenner, S.; Politi, Y., 1995: Dermatologic diseases and problems of women throughout the life cycle. International Journal of Dermatology 34(6): 369-379

Whitfield, C.L., 1995: The forgotten difference: ordinary memory versus traumatic memory. Consciousness and Cognition 4(1): 88-94

Goodall, W.N., 1962: A recent record of the Ovenbird in California. Condor, 64: 240-241