geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Identification and characterization of Marek's disease virus serotype 1 (MDV1) ICP22 gene product: MDV1 ICP22 transactivates the MDV1 ICP27 promoter synergistically with MDV1 ICP4


, : Identification and characterization of Marek's disease virus serotype 1 (MDV1) ICP22 gene product: MDV1 ICP22 transactivates the MDV1 ICP27 promoter synergistically with MDV1 ICP4. Veterinary Microbiology 85(4): 305-313

A previous report [Virus Genes 6 (1992) 365-378] has shown that the US1 gene of Marek's disease virus serotype 1 (MDV1) encodes a homologue of herpes simplex virus type 1 infected cell protein No. 22 (ICP22). In the present study, we expressed and identified a product of the MDV1 US1 gene in chicken embryo fibroblasts (CEFs) with the aid of a recombinant baculovirus expressing a Flag epitope-tagged MDV1 US1 gene, under control of the SRalpha promoter (composed of the enhancer region of the simian virus 40 early promoter and the R region of the human T-cell leukaemia virus type 1 long terminal repeat). In CEF infected with the recombinant baculovirus, MDV1 ICP22 was specifically and efficiently expressed in the presence of n-butyric acid. The apparent M(r) of the expressed protein was 30,000. Reporter gene assays revealed that MDV1 ICP22 by itself transactivated an MDV1 ICP27 promoter/reporter construct weakly but specifically, and furthermore, worked synergistically with MDV1 ICP4 to efficiently up-regulate the MDV1 ICP27 promoter. MDV1 ICP22 may be a regulatory protein that stimulates viral promoters in co-operation with other viral regulatory proteins such as MDV1 ICP4.

US$19.90

PMID: 11856580

DOI: 10.1016/s0378-1135(01)00522-3


Other references

Liau, C.-Suong; Lee, C.-Ming; Sheu, S.-Hsiung; Ueng, K.-Chang; Chien, K.-Liong; Su, T.-Chen; Lai, W.-Ter; Lin, M.-Cheng; Lin, C.-Sheng; Lin, C.-Sheng, 2005: Efficacy and safety of olmesartan in the treatment of mild-to-moderate essential hypertension in Chinese patients. Background and objective: Hypertension is very prevalent in the Chinese population in Taiwan. Chinese people frequently experience bothersome cough when receiving angiotensin-converting enzyme (ACE) inhibitors, and angiotensin II (AT(1)) receptor...

Earhart, R.W., 1958: The yellow chlorsis of oats re-appears in the Piedmont of South Carolina. The leading variety of oats currently grown in South Carolina appears to be particularly susceptible to this chlorosis.

Morris, C.S.; Edwards, M., 1943: Thermoduric organisms in milk. Heat-resistant types of organisms in milk can usually be traced to a lack of sanitation on the farm or inefficient cleaning of milk cans at the creamery. Heat resistant coliform organisms may come from dried films on utensils or from contaminated...

Mark D.S.evenson; D.K.m Rossmo; Robert J.K.ell; Steven C.L.C.mber, 2012: Geographic profiling as a novel spatial tool for targeting the control of invasive species. Geographic profiling (GP) was originally developed as a statistical tool in criminology, where it uses the spatial locations of linked crimes (for example murder, rape or arson) to identify areas that are most likely to include the offender's...

Nietfeldt J.W.; Ballinger R.E., 1989: A new method for storing animal tissue prior to mitochondrial dna extraction. Biotechniques 7(1): 31-32

Lilley S.G.; Florence T.M.; Stauber J.L., 1988: The use of sweat to monitor lead absorption through the skin. It is usually assumed that lead can be absorbed through the skin only if it is present as an organolead compound such as tetraethyllead or lead naphthanate. It has been found, however, that finely-powdered lead metal or lead nitrate solution place...

Natarajan, S., 2012: Pediatric ophthalmology: the oldest ophthalmology subspeciality. Indian Journal of Ophthalmology 59(6): 419-420

Chen, W.; Tang, H.; Zhou, M.; Hu, C.; Zhang, J.; Tang, K., 2015: Dexamethasone inhibits the differentiation of rat tendon stem cells into tenocytes by targeting the scleraxis gene. Glucocorticoid-induced tendon rupture is very common in clinical practice, and the overall outcome of surgical suture repair is rather poor. The mechanism remains unclear, and effective treatments are still lacking. In the present study, we invest...

Hall, D.A., 1947: The folic acid group of growth factors; the complexity of growth factors for Streptococcus lactis R. With the medium of Snell, Guirard and Williams (Abst. 1125, Vol. 12), a folic acid concentrate from spinach, the synthetic Lactobacillus casei factor, pteroylglutamic acid, vitamin BC various liver fractions, a poppy straw extract, thymine and oro...

Tagesson, C.; Chabiuk, D.; Axelson, O.; Barański, B.; Palus, J.; Wyszyńska, K., 1993: Increased urinary excretion of the oxidative DNA adduct, 8-hydroxydeoxyguanosine, as a possible early indicator of occupational cancer hazards in the asbestos, rubber, and azo-dye industries. Oxidative damage to DNA has been suggested to contribute to a number of diseases including cancer. In order to study the relationship between oxidative damage to DNA and occupational exposures, urinary excretion of the oxidative DNA adduct, 8-hydr...