+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Identification and characterization of an escorter for two secretory adhesins in Toxoplasma gondii

, : Identification and characterization of an escorter for two secretory adhesins in Toxoplasma gondii. Journal of Cell Biology ruary 5; 152(3): 563-578

The intracellular protozoan parasite Toxoplasma gondii shares with other members of the Apicomplexa a common set of apical structures involved in host cell invasion. Micronemes are apical secretory organelles releasing their contents upon contact with host cells. We have identified a transmembrane micronemal protein MIC6, which functions as an escorter for the accurate targeting of two soluble proteins MIC1 and MIC4 to the micronemes. Disruption of MIC1, MIC4, and MIC6 genes allowed us to precisely dissect their contribution in sorting processes. We have mapped domains on these proteins that determine complex formation and targeting to the organelle. MIC6 carries a sorting signal(s) in its cytoplasmic tail whereas its association with MIC1 involves a lumenal EGF-like domain. MIC4 binds directly to MIC1 and behaves as a passive cargo molecule. In contrast, MIC1 is linked to a quality control system and is absolutely required for the complex to leave the early compartments of the secretory pathway. MIC1 and MIC4 bind to host cells, and the existence of such a complex provides a plausible mechanism explaining how soluble adhesins act. We hypothesize that during invasion, MIC6 along with adhesins establishes a bridge between the host cell and the parasite.


PMID: 11157983

DOI: 10.1083/jcb.152.3.563

Other references

Wolfe, J.Liu.; Kawate, T.; Sarracino, D.A.; Zillmann, M.; Olson, J.; Stanton, V.P.; Verdine, G.L., 2002: A genotyping strategy based on incorporation and cleavage of chemically modified nucleotides. Aiming to facilitate the analysis of human genetic variations in the context of disease susceptibility and varied drug response, we have developed a genotyping method that entails incorporation of a chemically labile nucleotide by PCR followed by...

Vermilyea, S.G.; Huget, E.F.; de Simon, L.B., 1980: Apparent viscosities of setting elastomers. The flow behavior of five elastomeric impression materials was determined by extrusion viscometry. Initial viscosity ranged from 400 to 5,100 poise at a shear rate of 2.2 reciprocal seconds. All of the elastomers showed initial non-Newtonian behav...

Geetha, S.; Shetty, S.A.; Shetty, H.S.ekar; Prakash, H.S., 1996: Arachidonic acid-induced hypersensitive cell death as an assay of downy mildew resistance in pearl millet. Arachidonic acid (AA) induces hypersensitive response (HR) on coleoptile/root regions of two-day-old pearl millet seedlings. The response is comparable to the HR induced by the downy mildew pathogen, Sclerospora graminicola. A time gap in the appe...

Stephens, R.R.; Garrison, W.A., 2004: Firing mechanism for use in a surgical biopsy device. The invention described herein is an image-guided, vacuum assisted, percutaneous, coring, cable driven breast biopsy instrument which may be conveniently mounted to an x-ray machine. The biopsy instrument described herein incorporates incorporates...

Zerova, MD., 1998: The identity of Eurytoma spessivtsevi (Boucek et Novicky, 1956) to Decatomidea polygraphi (Ashmead, 1894) (Hymenoptera, Eurytomidae). Vestnik Zoologii. sentyabr'-dekabr'; 325-6: 32

Lundberg, S., 1969: Contributions to knowledge of the Swedish beetles. 12. Entomologisk tidskrift: 0 (3 4) 217-224

Semenov, V.; Mel'-Nik, I., 1977: The character of inheritance of some economic features in barley hybrids. Sbornik nauchnykh trudov: 1) 86-92

Whitby, H.; Jones, R.B., 1948: Farm incomes, rents and wages, 1939-40 to 1945-46. Financial details of 73 dairy farms situated throughout Scotland are given for the period 1939-40 to 1945-46. The farms are not completely representative of average conditions and have an average area of 266 acres with a rent of 18s. 3d. per acre....

Ünlü, Cüneyt.H.; Yapar, Gönül.; Erk, Çakıl., 2012: Eu(fod)3 binding on the 1H-NMR spectra of bis(2'-ethylbenzoate)ethylene glycol podands. The complexing and selective binding constants of Eu(fod)(3) with bis(2'-ethylbenzoate)ethylene glycol podands having one to four oxyethylene groups was observed on their (1)H-NMR spectra at 250 MHz and 295 K in CDCl(3). The Eu(fod)(3) in...

Walters, D.; Wilson, P.; Shuttleton, M., 1985: Relative changes in levels of polyamines and activities of their biosynthetic enzymes in barley infected with the powdery mildew fungus, Erysiphe graminis D.C. ex Merat f.sp. hordei Marchal. New phytologist 101(4): 695-705