+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Identification and characterization of stem cells in prepubertal spermatogenesis in mice

, : Identification and characterization of stem cells in prepubertal spermatogenesis in mice. Developmental Biology 258(1): 209-225

The stem cell properties of gonocytes and prospermatogonia at prepubertal stages are still largely unknown: it is not clear whether gonocytes and prospermatogonia are a special cell type or similar to adult undifferentiated spermatogonia. To characterize these cells, we have established transgenic mice carrying EGFP (enhanced green fluorescence protein) cDNA under control of an Oct4 18-kb genomic fragment containing the minimal promoter and proximal and distal enhancers; Oct4 is reported to be expressed in undifferentiated spermatogonia at prepubertal stages. Generation of transgenic mice enabled us to purify gonocytes and prospermatogonia from the somatic cells of the testis. Transplantation studies of testicular cells so far have been done with a mixture of germ cells and somatic cells. This is the first report that establishes how to purify germ cells from total testicular cells, enabling evaluation of cell-autonomous repopulating activity of a subpopulation of prospermatogonia. We show that prospermatogonia differ markedly from adult spermatogonia in both the size of the KIT-negative population and cell cycle characteristics. The GFP+ KIT- fraction of prospermatogonia has much higher repopulating activity than does the GFP+KIT+ population in the adult environment. Interestingly, the GFP+KIT+ population still exhibits repopulating activity, unlike adult KIT-positive spermatogonia. We also show that ALCAM, activated leukocyte cell adhesion molecule, is expressed transiently in gonocytes. Sertoli cells and myoid cells also express ALCAM at the same stage, suggesting that ALCAM may contribute to gonocyte-Sertoli cell adhesion and migration of gonoyctes toward the basement membrane.


PMID: 12781694

DOI: 10.1016/s0012-1606(03)00111-8

Other references

Ellis J.H.; Yuh Ming Lee, 1997: On the equivalence of kriging and maximum entropy estimators. For spatial interpolation and use in optimizing monitoring network design.

Hojo, T., 2002: Dental microwear of canines and M1s of late stone age compared with those of the modern age of Western Japan. American Journal of Physical Anthropology Supplement (34): 86

Bisbee, G.E.; Bachofer, H.J., 1979: AHA surveys major source of hospital data. The Annual Survey of Hospitals and the National Hospital Panel Survey work together to provide a thorough picture of hospital activities.

Godoy, B.A.; Gomes-Gouvêa, M.S.; Zagonel-Oliveira, M.; Alvarado-Mora, Mónica.V.; Salzano, F.M.; Pinho, Jão.R.R.; Fagundes, N.J.R., 2016: High prevalence of HBV/A1 subgenotype in native south Americans may be explained by recent economic developments in the Amazon. Infection, Genetics and Evolution 43: 354-363

Register, R.B.; Uncapher, C.R.; Naylor, A.M.; Lineberger, D.W.; Colonno, R.J., 1991: Human-murine chimeras of ICAM-1 identify amino acid residues critical for rhinovirus and antibody binding. Human ICAM-1 is the cellular receptor for the major group of human rhinoviruses (HRVs). Previous studies have suggested that the N-terminal domain of ICAM-1 is critical for binding of the major group rhinoviruses. To further define the residues wi...

Deck, K.B.; Kern, W.H., 1976: Local recurrence of breast cancer. Pathologic and surgical aspects of 1,027 breast cancer cases treated between 1961 and 1972 were reviewed. Sixty-eight (7%) of the cases recurred locally. This low rate of recurrence is associated with the small average diameter of 2.7 cm of all ca...

Schmidt, I., 1954: Diagnostic value of foveal entoptic phenomena in glaucoma. A.M.A. Archives of Ophthalmology 52(4): 583-597

Choi, K.M.; Lee, J.; Kim, Y.H.; Kim, K.B.; Kim, D.L.; Kim, S.G.; Shin, D.H.; Kim, N.H.; Park, I.B.; Choi, D.S.; Baik, S.H., 2003: Relation between insulin resistance and hematological parameters in elderly Koreans-Southwest Seoul (SWS) Study. In this study, we investigated the relation between insulin resistance and hematological parameters in elderly Koreans. This study included 1314 non-diabetic subjects over the age of 60, selected from a cross-sectional study, which was conducted i...

Mason, M.J.; Mainwood, G.W.; Thoden, J.S., 1986: The influence of extracellular buffer concentration and propionate on lactate efflux from frog muscle. Lactate efflux from frog sartorius muscles was measured following a lactate load of about 18 mumol X g-1 induced by a 4-min period of stimulation. Lactate efflux rate was buffer concentration dependent. The initial efflux rate increased from about...

Schulte-Sienbeck, H., 1974: Suggestions concerning feed quality for the mixed feed manufacturer. Kraftfutter 57 (11) 480