+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Identification and chromosomal organization of two rye genome-specific RAPD products useful as introgression markers in wheat

, : Identification and chromosomal organization of two rye genome-specific RAPD products useful as introgression markers in wheat. Genome 45(1): 157-164

Two rye genome-specific random amplified polymorphic DNA (RAPD) markers were identified for detection of rye introgression in wheat. Both markers were amplified in all of the tested materials that contained rye chromatin such as rye, hexaploid triticale, wheat-rye addition lines, and wheat varieties with 1BL.1RS translocation. Two cloned markers, designated pSc10C and pSc20H, were 1012 bp and 1494 bp, respectively. Sequence analysis showed that both pSc10C and pSc20H fragments were related to retrotransposons, ubiquitously distributed in plant genomes. Using fluorescence in situ hybridization (FISH), probe pSc10C was shown to hybridize predominantly to the pericentromeric regions of all rye chromosomes, whereas probe pSc20H was dispersed throughout the rye genome except at telomeric regions and nucleolar organizing regions. The FISH patterns showed that the two markers should be useful to select or track all wheat-rye translocation lines derived from the whole arms of rye chromosomes, as well as to characterize the positions of the translocation breakpoints generated in the proximal and distal regions of rye arms.


PMID: 11908658

DOI: 10.1139/g01-133

Other references

Schaad N.C.; Schorderet M.; Magistretti P.J., 1989: Modulation of vip stimulated cyclic amp formation reveals three classes of excitatory amino acids eaa responses in mouse cerebral cortical slices. Society for Neuroscience Abstracts 15(2): 1161

Lornudd, C.; Bergman, D.; Sandahl, C.; von Thiele Schwarz, U., 2016: Healthcare managers' leadership profiles in relation to perceptions of work stressors and stress. Purpose The purpose of this study is to investigate the relationship between leadership profiles and differences in managers' own levels of work stress symptoms and perceptions of work stressors causing stress. Design/methodology/approach Cro...

Zweiman, B.; Levinson, A.I., 1988: Immunologic aspects of neurologic diseases. I. General responses and demyelinating diseases. Journal of Allergy and Clinical Immunology 81(6): 1067-1077

Dobsíková, R.; Velísek, J.; Wlasow, T.; Gomulka, P.; Svobodová, Z.; Novotný, L., 2006: Effects of cypermethrin on some haematological, biochemical and histopathological parameters of common carp (Cyprinus carpio L.). The study aimed at assessing the effects of a pyrethroid cypermethrin on common carp (Cyprinus carpio L.) on the basis of the results of acute toxicity tests and haematological, biochemical and histological examinations. Experimental carp were exp...

Buddenbrock, W.; Friedrich, H., 1932: Uber Pallreflexe von Arthropoden. Zoologische Jahrbuecher Jena (Allgemeine Zoologie) Bd, 51: 131-148

Hori, S., 2001: Human body burden and intake of PCDDs, PCDFs and Co-PCBs in breast-fed infants and adults of Japan. Intake and body burden of the dioxins (PCCDs, PCDFs and Co-PCBs) were investigated in breast-fed infants and adults using data from several Prefectures. Levels in infants were similar in the different Prefectures. Levels reached 61.4 pg/g fat toxi...

Shahein, A.H.; Agwah, E.M.R.; E.S.ammah, H.A., 1995: Effect of plant density as well as nitrogen and phosphorus fertilizer rate on growth, green pods and dry seed yield and quality of broad bean. Field trials were conducted in Egypt in 1992-94 to assess the effects of 0-40 kg N and 0-45 kg P2O5/feddan on Vicia faba cv. Aquadulce sown on one or both sides of ridges 60 cm wide, 4 m long and 30 cm apart. Sowing on both sides of ridges reduced...

Shacter, B., 1956: Interrelations in respiratory, phosphorylative, and mitotic activity of Ehrlich ascites-tumor cells; influence of stilbestrol and quinone. Effects of graded concentrations of diethylstil-bestrol and benzoquinone on interrelationships between respiration and phosphorylations of Ehrlich ascites-tumor cells were studied in vitro. With increasing concentration, both reagents progressivel...

Garrahan, T.B.; Sinclair, S.A.; McCormick, W.W., 1986: A market niche for wooden pallet bins. A discussion on the use of pallet-mounted wooden containers by 150 vegetable processors in the Eastern USA who responded to a survey. There appears to be a potential market for leasing bins.

Tanabe, Kazushige., 1994: Primitiveness of the chambered Nautilus (Mollusca; Cephalopoda) from the viewpoint of comparative embryology. Fossils (Tokyo). June 30; 56: 42-46