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Identification and cloning of Schistosoma japonicum novel genes - III 8kDa calcium-binding protein gene


, : Identification and cloning of Schistosoma japonicum novel genes - III 8kDa calcium-binding protein gene. Journal of Tropical Medicine Guangzhou 2(2): 114-117

The III 8-kDa calcium-binding protein (CBP) cDNA of Schistosoma japonicum was subcloned into the expression plasmid pET32a(+) to investigate its function. The insert cDNA fragment was sequenced and analysed using the BLASTn programme. The DNA sequence was highly homologous to the S. mansoni 8-kDa calcium-binding protein cDNA. Two PCR primers were designed according to this CBP cDNA sequence and the cloning site in the pET32a(+) plasmid.

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