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Identification and cloning of a key insecticide-metabolizing glutathione S-transferase (MdGST-6A) from a hyper insecticide-resistant strain of the housefly Musca domestica

, : Identification and cloning of a key insecticide-metabolizing glutathione S-transferase (MdGST-6A) from a hyper insecticide-resistant strain of the housefly Musca domestica. Insect Biochemistry and Molecular Biology 31(12): 1145-1153

Strains of the housefly, Musca domestica, highly resistant to organophosphate (OP) and other insecticides are known because they overproduce glutathione S-transferases (GSTs). Previous work has shown that overproduction in these strains involved numerous isozymes with glutathione conjugating activities (Pesticide Biochem. Physiol., 25 (1986) 169; Mol. General Genetics, 227 (1991) 355; J. Biol. Chem., 267 (1992) 1840; Mol. General Genetics, 245 (1994) 236; J. Mol. Evol., 43 (1996) 236). The current work describes the purification and identification of a M. domestica GST isozyme (pI 7.1) broadly specific for substrates from a housefly strain, Cornell-HR, that is highly resistant against OP-insecticides, and the isolation of two new MdGST genes using the antibody made against it. This isozyme, which was identified from amongst more than 20 isoelectric forms of GSTs of the same subunit size, was highly active for conjugating GSH to the model substrate 3,4-dichloronitrobenzne (DCNB). When expressed in Escherichia coli, one of the cloned GSTs, MdGST-6A, produces an enzyme that conjugates glutathione to the insecticides methyl parathion and lindane. On indication that it was the most active isozyme toward several xenobiotics among several MdGSTs tested, we advance the notion that MdGST-6A probably plays an important role in M. domestica Cornell-HR's resistance towards OP-insecticides. MdGST-6A and a second closely related one found in this work, MdGST-6B, are members of the traditional insect class I family (theta-class) and share the greatest homologies with a cluster of Drosophila GSTs on locus 55. In addition to having the unusually broad substrate specificity, the sequence of the new group of enzymes reveals that it has a highly diverged hydrophobic motif in its active site as compared to other class I GSTs from insects.


PMID: 11583927

DOI: 10.1016/s0965-1748(01)00059-5

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Kobashi, S.; Kubo, H.; Yamauchi, T.; Higashiyama, K., 2002: Antinociceptive effects of 1-acyl-4-dialkylaminopiperidine and 1-alkyl-4-dialkylaminopiperidine in mice: structure-activity relation study of matrine-type alkaloids. We previously reported that (+)-matrine and (+)-allomatrine have antinociceptive properties mediated mainly through the activation of kappa-opioid receptors. 1-Acyl-4-dialkylaminopiperidines were synthesized as the simplest derivatives of matrine,...

Stevenson T.H., 1972: Evidence of Yarmouth dissection and mass-wastage between Seward and Lincoln, Nebraska. Proceedings of the Nebraska Academy of Sciences and Affiliated Societies 82: 43-44

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von Elzenbaum, H., 1968: Protection of esophago-jejunal anastomosis by a pedicled sero-muscular sleeve. La Presse Medicale 76(28): 1433-1433

Fritz Peter; Krouse H.R., 1972: Thermal pollution and water balance of Wabamun Lake; an isotopic investigation. Eos, Transactions, American Geophysical Union 53(4): 378

Baier, W.K., 1980: Interference of cortisone and ACTH with brain development? A survey of experimental studies in mice and rats. The tumoral and cerebrospinal fluid (CSF) levels of cyclic nucleotides and of the main adrenergic metabolites (homovanillic acid and 5-hydroxyindoleacaetic acid) were investigated in a group of children with posterior fossa medulloblastomas. A lon...

Nellemann, G., 1978: A registration and retrieval system for private literature. Ugeskrift for Laeger 140(13): 744-745

Klapste, J., 1984: Albinistic feathers in a marsh harrier. Australian Bird Watcher, 105: 161-163