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Identification and detection of carnation mottle virus on carnation by RT-PCR


, : Identification and detection of carnation mottle virus on carnation by RT-PCR. Plant Protection 28(1): 8

A virus isolate was obtained from diseased carnation (Dianthus caryophyllus) showing symptoms of mottled leaves and split flowers. By observation with TEM the virus particle was shown to be spherical of about 28-33 nm in diameter. The purified virus from infected tissues had a typical ultraviolet absorption curve of nucleoprotein, i.e. OD260/OD280 = 1.7.

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