geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Identification and differentiation of Leishmania species in clinical samples by PCR amplification of the miniexon sequence and subsequent restriction fragment length polymorphism analysis


, : Identification and differentiation of Leishmania species in clinical samples by PCR amplification of the miniexon sequence and subsequent restriction fragment length polymorphism analysis. Journal of Clinical Microbiology 41(7): 3147-3153

We recently developed a new PCR-restriction fragment length polymorphism (RFLP)-based assay using the miniexon sequence from the genus Leishmania. Here we report the application of this new genotyping method to naturally infected clinical samples for the differentiation of New and Old World Leishmania species. Of the newly developed assay and four currently applied diagnostic tests (i.e., in vitro cultivation, serology, and two other molecular assays using either the small subunit-internal transcribed spacer sequence or a repetitive genomic sequence), the miniexon assay showed the highest sensitivity, 89.7%, compared to 70.6, 57.1, 51.7, and 79.3%, respectively. Species differentiation was robust and reliable compared with that by two other Leishmania genotyping techniques. The assay provides a valuable tool for the identification of Leishmania directly from clinical samples and enables determination of the infecting species by a facile technique with high discrimination power. Since Leishmania causes a broad spectrum of diseases distinguished by different parasite and host factors, detection and characterization of the infecting species is crucial for the confirmation of a diagnosis as well as the establishment of the clinical prognosis and the initiation of an adequate therapeutic approach. The miniexon PCR-RFLP assay will facilitate such determination and might improve diagnosis and treatment of leishmaniasis.

US$19.90

PMID: 12843055

DOI: 10.1128/jcm.41.7.3147-3153.2003


Other references

Abdul Aziz, G.M.; E.T.lty, Y.E.; Ali, M.A., 1997: Biological treatments of straws in animal nutrition. Chopped rice straw (RS) and bean straw (BS) were assigned to one of four treatments. Treatments one (T1) with T. viride while treatment two (T2) with T. viride and Saccharomyces cerevisiae. The straws were previously autoclaved at 121 degrees C fo...

Adler, C.H., 2009: Perioperative use of botulinum toxins. Botulinum toxins are not commonly thought of as adjuncts to surgical procedures. This article reviews some of the perioperative uses of botulinum toxins.

Galimov, O.V.; Prazdnikov, E.N., 1993: Surgical management of gastroduodenal ulcer associated with hiatal hernia. The problem of surgical management of gastric and duodenal ulcers associated with the hiatal esophageal hernias is reported. The authors have summed up the results of simultaneous surgical treatment of 97 patients. Good follow-up results were obta...

Sanada, J.; Nakamura, K.; Minami, Y.; Kuroiwa, N.; Ohshige, T.; Hashimoto, S., 1983: Assessment of left ventricular isovolumetric contraction flow by the use of pulsed Doppler echocardiography. Kokyu to Junkan. Respiration & Circulation 31(1): 81-87

Martin Gerald P.R., 1951: Vulkanische Aktivitaet kein Klima- Factor. Concludes that only short-range optical and radiational effects have been caused by explosive volcanic action in the geologic past, and that the origin ofice ages is not related to volcanism.

Camiña, M.F.; Rozas, I.; Gómez, M.; Paz, J.M.; Alonso, C.; Rodriguez-Segade, S., 1991: Short-term effects of administration of anticonvulsant drugs on free carnitine and acylcarnitine in mouse serum and tissues. 1. The short-term evolution of concentrations of free carnitine and acylcarnitine was studied in the serum, liver, kidney, heart and skeletal muscle of mice after administration of single therapeutic doses of the anticonvulsant drugs, valproic aci...

Carlon N.; Stahl A.; D.L.nversin A., 1970: Volumetric and cytologic variations of the intermediate lobe of the pituitary of the rat after administration of metopirone. Comptes Rendus des Seances de la Societe de Biologie et de ses Filiales 164(8-9): 1775-1782

Buemann, B.; Toubro, S.; Raben, A.; Astrup, A., 1999: Human tolerance to a single, high dose of D-tagatose. In a double-blind randomized cross-over study, human subjects consumed a continental breakfast sweetened with 29 g D-tagatose or sucrose. Findings indicated that 29 g D-tagatose seems to be tolerated without unacceptable side effects in most indiv...

Lee, H.Ji.; Jin, J.; Shen, S., 2003: A study of the licensing system in Korean Oriental medicine. In 1952, the Licensing System of Korean Oriental Practitioners was established by law and practiced until now. It was the most important aspect of the Professionalization of Korean Oriental medicine, and it also affected other aspects of the Profe...

Teasdale, G., 2014: Achieving success in reducing teenage pregnancy in Hull. Perspectives in Public Health 134(5): 292-296