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Identification and expression analysis of hepcidin-like antimicrobial peptides in bony fish


, : Identification and expression analysis of hepcidin-like antimicrobial peptides in bony fish. Developmental and Comparative Immunology e- y; 27(6-7): 589-601

Antimicrobial peptides play a crucial role as the first line of defense against invading pathogens. Several types of antimicrobial peptides have been isolated from fish, mostly of the cationic -helical variety. Here, we present the cDNA sequences of five highly disulphide-bonded hepcidin-like peptides from winter flounder, Pseudopleuronectes americanus (Walbaum) and two from Atlantic salmon, Salmo salar (L.). These hepcidin-like molecules consist of a 24 amino acid signal peptide and an acidic propiece of 38[long dash]40 amino acids in addition to the mature processed peptide of 19[long dash]27 amino acids. Exhaustive data mining of GenBank with these sequences revealed that similar peptides are encoded in the genomes of Japanese flounder, rainbow trout, hybrid striped bass and medaka, indicating that they are widespread among fish. Southern hybridization analysis suggests that closely related hepcidin-like genes are present in other flatfish species, and that they exist as a multigene family clustered on the winter flounder genome. Hepcidin variants are differentially expressed during bacterial challenge, during larval development of P. americanus and in different tissues of adult fish.

US$19.90

PMID: 12697315

DOI: 10.1016/s0145-305x(03)00036-3


Other references

Vecchio, R.; Ferrara, M.; Mosca, F.; Messina, G.; Ignoto, A.; D.M.rtino, M.; Latteri, F., 1995: Role of thymopentin in the prevention of post-splenectomy infections. The Authors evaluated the efficacy of thymopentin in the prevention of post-splenectomy infections. 14 splenectomized patients treated by thymopentin (50 mg s.c. every other day in the 4 weeks following splenectomy) were compared with 14 splenecto...

Kamenov, G., 1970: Effect of soil moisture upon water exchange in the tissues and upon the productivity of some hybrid maize cultivars. Plants of the hybrids VIR42 and Wisconsin 641AA contained greater amounts of bound water and were characterized by greater water retention and lower water activity compared with later-maturing Ohio C92 and Kansas 1859. The lower water activity was...

Jørgensen, M.Eika., 2004: Glucose intolerance and its relation to cardiovascular risk factors among Greenland Inuit. The Greenland Population Study. International Journal of Circumpolar Health 63(3): 286-288

Aucamp, J.D., 1970: Old man saltbush: method of establishment. The best method of establishing old man saltbush (Atriplex nummularia) in areas with annual rainfall as low as 6-8 in. was by planting 5- to 7-month-old seedlings 3-6 ft apart in rows 6 ft apart, with 3 irrigations/year in the planting and 1st har...

Mahata, S., K.; Gruber, B.; Mahata, M.; Roeder, C.; Fischer-Colbrie, R.; Sperk, G., 1993: Kainic acid seizures in the rat: Differential expression of chromogranin A, carboxypeptidase H and peptidylglycine alpha-amidating monooxygenase in subfields of the hippocampal formation. Using in situ hybridization histochemistry concentrations of mRNAs encoding chromogranin A (ChA), carboxypeptidase H (CPH) and peptidylglycine alpha-amidating monooxigenase (PAM) have been investigated in the hippocampus after kainic acid (KA)indu...

Labit L.B.uteiller, C.; Astruc, R.; Minty, A.; Ferrara, P.; Lupker, J.H., 1995: Isolation of an IL-13-dependent subclone of the B9 cell line useful for the estimation of human IL-13 bioactivity. A novel sub-clone of the B9 hybridoma cell line (B9-1-3) has been selected by cloning following continuous culture in rhIL-13. This cell line shows an increased sensitivity to both hIL-13 and mIL-4 compared to the parental B9 cell line. The prolif...

Kurata, Y.; Hayashi, S.; Oshida, M.; Kiyoi, T.; Tadokoro, S.; Kashiwagi, H.; Honda, S.; Tomiyama, Y., 2000: The sensitivity, specificity and predictive value of PAIgG, reticulated platelets, thrombopoietin levels, and platelet size for the differential diagnosis of thrombocytopenia. We evaluated measurements of PAIgG, reticulated platelets (RP), plasma thrombopoietin (TPO) levels, and platelet size to determine whether these parameters were useful for the differential diagnosis of idiopathic thrombocytopenic purpura (ITP), ap...

Lutz, S., 1993: Methodist offers early retirements. Modern Healthcare 23(38): 8-8

Blockman, M., 2006: Selective serotonin reuptake inhibitors in children with major depression. South African Medical Journal 96(6): 476-7; Author Reply 477-8

Norman, M.G.; Taylor, G.P.; Clarke, L.A., 1990: Sudden, unexpected, natural death in childhood. One thousand nine hundred and fifty four autopsies performed at British Columbia's Children's Hospital during a 7-year period were reviewed to determine the causes of sudden unexpected natural death in the age group from birth to 17 year...