+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Identification and functional validation of a 5' upstream regulatory sequence in the human tyrosinase gene homologous to the locus control region of the mouse tyrosinase gene

, : Identification and functional validation of a 5' upstream regulatory sequence in the human tyrosinase gene homologous to the locus control region of the mouse tyrosinase gene. Pigment Cell Research 16(6): 685-692, December

Comparison analysis of the sequences of the mouse and human genomes has proven a powerful approach in identifying functional regulatory elements within the non-coding regions that are conserved through evolution between homologous mammalian loci. Here, we applied computational analysis to identify regions of homology in the 5' upstream sequences of the human tyrosinase gene, similar to the locus control region (LCR) of the mouse tyrosinase gene, located at -15 kb. We detected several stretches of homology within the first 30 kb 5' tyrosinase gene upstream sequences of both species that include the proximal promoter sequences, the genomic region surrounding the mouse LCR, and further upstream segments. We cloned and sequenced a 5' upstream regulatory sequence found between -8 and -10 kb of the human tyrosinase locus (termed h5'URS) homologous to the mouse LCR sequences, and confirmed the presence of putative binding sites at -9 kb, homologous to those described in the mouse tyrosinase LCR core. Finally, we functionally validated the presence of a tissue-specific enhancer in the h5'URS by transient transfection analysis in human and mouse cells, as compared with homologous DNA sequences from the mouse tyrosinase locus. Future experiments in cells and transgenic animals will help us to understand the in vivo relevance of this newly described h5'URS sequence as a potentially important regulatory element for the correct expression of the human tyrosinase gene.


PMID: 14629727

DOI: 10.1046/j.1600-0749.2003.00100.x

Other references

Soy, D.; Lopez, M.C.; Salvador, L.; Parra, L.; Roca, M.; Chabas, E.; Codina, C.; Modamio, P.; Mariño, E.L.; Ribas, J., 1994: Stability of an oral midazolam solution for premedication in paediatric patients. The objectives of this study were to assess the stability of a 1 mg/ml oral midazolam solution elaborated by our Hospital Pharmacy Service, and to confirm its clinical effect in presurgical paediatric patients. The solution's stability was te...

Lucks, J.B.; Qi, L.; Mutalik, V.K.; Wang, D.; Arkin, A.P., 2011: Versatile RNA-sensing transcriptional regulators for engineering genetic networks. The widespread natural ability of RNA to sense small molecules and regulate genes has become an important tool for synthetic biology in applications as diverse as environmental sensing and metabolic engineering. Previous work in RNA synthetic biol...

Malur M.N., 1992: Lithostratigraphy and depositional environment of Niniyur sediments (Paleocene), Tiruchirapalli area, southern India. International Geological Congress, Abstracts Congres Geologique Internationale, Resumes: ages 318. 1992.

Wagstaff, J.; Jonas, M.M., 1995: Closing in on a "familial cholestasis gene". Hepatology 22(5): 1611-1613

Koshkina, Aa, 1977: Effectiveness of selection of Kholmogor cows according to origin and inherent productivity. Zhivotnovodstvo 3 32-34

Yasue, H., 1995: 2-Acrylamine-2-methyl-1-propanesulfonic acid enhancement of alkaline phosphatase label detection. Official Gazette of the United States Patent & Trademark Office Patents 1177(4): 2496, Aug 22

Wang, X.Z.; Wu, Z.Y.; Zhao, S.R., 1982: Comprehensive control of waterlogging and salinization injury to crops in areas with patches of alkali soil north of the Huaihe River, Jiangsu Province. Field trials and pot-test data indicated that crop growth could be improved by drainage, deep ploughing and the application of manure.

Lai, S.W.; Lin, H.C.; Lin, C.C.; Liu, C.S.; Shih, Y.T.; Peng, C.T.; Tsai, C.H.; Tsai, F.J.; Ng, K.C., 2000: Clinical analysis of a dysentery outbreak in Taichung. In September 1993, we collected 207 patients due to dysentery, who visited the Department of Pediatrics at China Medical College Hospital. In our report, 67.6% of these patients were amebic dysentery, 19.3% were combined infection with amebic and...

Laemmler, G., 1964: Experimental Chemotherapy Of Trematode Infections And The Related Problems. 2. Zeitschrift für Tropenmedizin und Parasitologie 15: 165-199

Wilde, P.; Quinby-Hunt, MS.; Berry, WBN., 1990: Vertical advection from oxic or anoxic water from the main pycnodine as a cause of rapid extinction or rapid radiations. Lecture Notes in Earth Sciences, 30: 85-98