geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Identification and isolation of two ascomycete fungi from spores of the arbuscular mycorrhizal fungus Scutellospora castanea


, : Identification and isolation of two ascomycete fungi from spores of the arbuscular mycorrhizal fungus Scutellospora castanea. Applied and Environmental Microbiology 68(9): 4567-4573

Two filamentous fungi with different phenotypes were isolated from crushed healthy spores or perforated dead spores of the arbuscular mycorrhizal fungus (AMF) Scutellospora castanea. Based on comparative sequence analysis of 5.8S ribosomal DNA and internal transcribed spacer fragments, one isolate, obtained from perforated dead spores only, was assigned to the genus Nectria, and the second, obtained from both healthy and dead spores, was assigned to Leptosphaeria, a genus that also contains pathogens of plants in the Brassicaceae. PCR and randomly amplified polymorphic DNA-PCR analyses, however, did not indicate similarities between pathogens and the isolate. The presence of the two isolates in both healthy spores and perforated dead spores of S. castanea was finally confirmed by transmission electron microscopy by using distinctive characteristics of the isolates and S. castanea. The role of this fungus in S. castanea spores remains unclear, but the results serve as a strong warning that sequences obtained from apparently healthy AMF spores cannot be presumed to be of glomalean origin and that this could present problems for studies on AMF genes.

US$19.90

PMID: 12200315

DOI: 10.1128/aem.68.9.4567-4573.2002


Other references

Kim, J.Hun.; Kim, J.Hong.; Han, J.Ho.; Yoo, B.Moo.; Kim, M.Wook.; Kim, W.Hwan., 2009: Is endoscopic papillectomy safe for ampullary adenomas with high-grade dysplasia?. This study was designed to provide safe management guidelines for ampullary adenoma by analysis of clinicopathological features. The treatment of ampullary cancer has been established; however, the indications for treatment of ampullary adenoma re...

Rimini, R., 1958: Functional rehabilitation of the diaphragm in dyspneic emphysematous patients. Hoja Tisiologica 18(2): 100-113

McConnell, V.C.; Ritchie, B.W., 2000: Calculations for the veterinary professional. The following topics are covered in this manual: fractions and decimals; scientific notation; temperature and units of volume; equations, ratio and proportion, and physical chemistry; dilution and drug dosing by body surface area; i.v. drips, oral...

Hashim, P.W.; Hashim, S.W., 2014: Aortic cannulation with a single purse-string suture. Journal of Thoracic and Cardiovascular Surgery 147(4): 1430-1431

Mondini, S., 1972: The problem of additives in animal production. Annali 92 (2) 199-202

Lavi, O.; Ginsberg, D.; Louzoun, Y., 2012: Regulation of modular Cyclin and CDK feedback loops by an E2F transcription oscillator in the mammalian cell cycle. The cell cycle is regulated by a large number of enzymes and transcription factors. We have developed a modular description of the cell cycle, based on a set of interleaved modular feedback loops, each leading to a cyclic behavior. The slowest loo...

Barros, S.V. da S.G. de; Carlo, R.J. del; Viloria, M.I.; Galvao, S.R.; Maia Filho, A.; Oliveira, D.R., 2001: Percutaneous bone marrow autograft. II. Repair of segmentar defects produced in the radii rabbit. The whole and concentrated bone marrow percutaneous autografts used to repair osseous defects were investigated. 16 male New Zealand White rabbits, aged between 5 and 6 months with average body weight of 3.5 kg were divided into groups I and II. I...

Thorpe, A., 2004: The nature and causes of poverty: an overview. This chapter first shows how a narrow definition of poverty related to consumptive shortcomings can be amplified to accommodate shortfalls in access to shelter and other basic needs. These shortfalls can, in turn, be monetized allowing the computa...

Zhu, Y.; Zhou, X.Y.; Yao, X.D.; Dai, B.; Ye, D.W., 2007: The prognostic significance of p53, Ki-67, epithelial cadherin and matrix metalloproteinase-9 in penile squamous cell carcinoma treated with surgery. To evaluate the prognostic significance of p53, Ki-67, epithelial cadherin (E-cadherin) and matrix metalloproteinase-9 in primary penile cancer, as the presence of lymph node metastasis and long-term survival are hard to define in penile squamous...

Martin, F.; Dubach, P.; Mehta, N.C.; E.A., 1963: Determination of functional groups of humic substances.. In the tetrahydrofuran-soluble fraction of fulvic acid from the Bh horizon of a podzol, determinations were made of -COOH groups (4.8-7.5 meq/g) by redaction with diboran (B2H6), -OH groups (3.6 meq/g) by acetylation, & -CO groups (1 meq/g) by mea...