geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Identification and localization of a neprilysin-like activity that degrades tachykinin-related peptides in the brain of the cockroach, Leucophaea maderae, and locust, Locusta migratoria


, : Identification and localization of a neprilysin-like activity that degrades tachykinin-related peptides in the brain of the cockroach, Leucophaea maderae, and locust, Locusta migratoria. Journal of Comparative Neurology 457(1): 57-66

Neprilysin (NEP) is an endopeptidase, which has an important role in the inactivation of mammalian tachykinins. NEP-like activity has also been found in the brain of several insects; however, the lack of information about the cellular localization of this peptidase has hindered our understanding of its role in peptidergic signaling in insects. We now provide evidence that membrane-bound NEP is involved in the inactivation of tachykinin-related peptides in the brain of the cockroach, Leucophaea maderae, and the locust, Locusta migratoria. The L. maderae enzyme cleaved the cockroach peptide LemTRP-1 and the mammalian NEP substrate [DAla(2),Leu(5)]enkephalin at the Gly-Phe peptide bond. The enzyme was acted upon by the NEP inhibitors phosphoramidon (IC(50), 0.64 microM) and thiorphan (IC(50), 1.23 microM), and the detergent-solubilized enzyme had an Mr of approximately 300,000 and a neutral pH optimum. This endopeptidase cleaved another insect tachykinin-related peptide, CavTK-II, in a predictable manner at the Ala-Phe peptide bond, suggesting that the peptidase can hydrolyse tachykinin-related peptides with different structures. NEP activity was histochemically localized in several, but not all, regions of neuropil in the brain of L. maderae, including the central body, the lobula of the optic lobe, and the tritocerebrum. All of these regions are known to receive neuronal processes containing tachykinin-related peptides. A slightly different distribution pattern for NEP was observed in the brain of L. migratoria. Again, NEP was localized to regions of the neuropil that also display tachykinin-related peptide immunoreactivity. The data reported provide evidence for an evolutionary conserved role for NEP in the inactivation of tachykinin-related peptides in the brain.

US$19.90

PMID: 12541324

DOI: 10.1002/cne.10561


Other references

Bairden, K.; Armour, J.; McWilliam, P.N., 1985: Trichostrongyle larval populations on herbage from grazed and ungrazed cattle pastures in south west Scotland. In the course of studies on the control of bovine parasitic gastroenteritis in south west Scotland using a morantel sustained release bolus the populations of trichostrongyle infective larvae on herbage were monitored over a two-year period. From...

Chin, J-Hyang.; Shiwaku, H.; Goda, O.; Komuro, A.; Okazawa, H., 2009: Neural stem cells express Oct-3/4. There remain controversy and disagreement on whether Oct-3/4 is expressed in neural stem cells or not. Although many reports had shown expression of Oct-3/4 in somatic stem and progenitor cells, conditional KO mice of Oct-3/4 in neural stem cells...

Vorbrodt A.; Lambiotte M.; Benedetti E.L., 1974: Cytochemical and ultrastructural studies on the formation of bile canaliculi in rat fetal hepatocytes cultivated in vitro. Folia Histochemica et Cytochemica 12(3/4): 358-359

Crome, Francis H.J., 1997: Researching tropical forest fragmentation Shall we keep on doing what were doing?. Laurance, W F , Bierregaard, R O , Jr Tropical forest remnants: Ecology, management, and conservation of fragmented communities : 485-501

Varga, A., 1978: Neuer Fundort von Trichia lubomirskii (Slosarski) in Ungarn. Soosiana, 6: 31-34

Kerut, E.Kenneth.; Hanawalt, C.; Everson, C.T., 2007: Role of the echocardiography laboratory in diagnosis and management of pacemaker and implantable cardiac defibrillator infection. Echocardiography 24(9): 1008-1012

Sciortino, A.D.; Carlton, D.C.; Axelrod, A.; Eng, M.; Zhukovsky, D.S.; Vinciguerra, V., 1993: The efficacy of administering blood transfusions at home to terminally ill cancer patients. Comfort care for cancer patients in the terminal stage has been the goal of hospice programs since their inception. Among the many treatment modalities available to homebound patients, transfusions have been little studied for their effects on adv...

Marotta, U., 1955: Roentgenographic method for the determination of calcium in bones. Rendiconti - Istituto Superiore di Sanita 18(6): 463-476

Ruiz Mirazo Kepa, 2006: Working towards the minimal cell; a bottom-up proposal. Origins of Life and Evolution of the Biosphere 36(3): 216-218

Cooksey, R.C.; Facklam, R.R.; Thornsberry, C., 1978: Antimicrobial susceptibility patterns of Streptococcus pneumoniae. Fifty clinical isolates of Streptococcus pneumoniae received by the Streptococcus Laboratory of the Center for Disease Control from August 1976 through March 1977 and 50 pneumococcal strains retrieved from 13- to 16-year storage (originally isolat...