+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Identification and mapping of the promoter for the gene encoding the ferritin heavy-chain homologue of the yellow fever mosquito Aedes aegypti

, : Identification and mapping of the promoter for the gene encoding the ferritin heavy-chain homologue of the yellow fever mosquito Aedes aegypti. Insect biochemistry and molecular biology 33(1): 51-62

Mosquitoes are responsible for the transmission of numerous human diseases. The recent development of transgenic mosquitoes provides a new tool to examine molecular interactions between insect vectors and the pathogens they transmit. One focus in generating transgenic mosquito lies on expressing anti-pathogenic proteins at primary sites of pathogenic invasions, specifically the mosquito gut. Promoters that direct the expression of anti-pathogenic proteins in the mosquito gut are thus sought after because they may provide ways to hinder pathogenic development in the mosquito. Here, we report the identification and mapping of a strong promoter from the Aedes aegypti ferritin heavy-chain homologue (HCH) gene. All known insect ferritin HCH genes are expressed in the gut and inducible by an iron overload. Our transfection assays and DNase I footprinting analyses show that the mosquito ferritin HCH-gene contains regulatory elements both upstream and downstream of the transcriptional start site. The promoter of this gene contains a CF2 site, two GATA-binding sites, an E2F site, a TATA-box, an AP-1 site and a C/EBP binding site.


DOI: 10.1016/s0965-1748(02)00167-4

Other references

McLaughlin, D.B., 2016: Drug Prices: A Policy Puzzle. Healthcare Executive 31(2): 68, 70-1

Pulakat, L.; Cooper, S.; Knowle, D.; Mandavia, C.; Bruhl, S.; Hetrick, M.; Gavini, N., 2005: Ligand-dependent complex formation between the Angiotensin II receptor subtype AT2 and Na+/H+ exchanger NHE6 in mammalian cells. Involvement of Angiotensin II (Ang II) in the regulation of sodium levels by modulating the Na+/H+ exchangers is demonstrated in many tissues. Screening of a mouse 17-day fetus cDNA library with the Angiotensin II receptor AT2 as the bait in yeast...

Mander, R., 2001: Response to: Male appropriation and medicalization of childbirth; an historical analysis by H.A. Churchill (2001) Journal of Advanced Nursing 33, 334-342. Journal of Advanced Nursing 35(3): 390-391

Oldshue, J.Y., 1966: Fermentation mixing scale-up techniques. Scale-up of a mixing process in fermentation involves breaking the process down into individual but interrelated steps. The effect of mixing on gas-liquid absorption, fluid shear rates, blending, and heat transfer allows each to be considered sepa...

Meyer, C.; Wiese-Posselt, M.; Deleré, Y.; Reiter, S.; Koch Institute; Ständigen Impfkommission am Robert Koch-Institut, 2007: Recommendations of the Vaccine Standards Commission of the Robert Koch Institute. Pharmazie in Unserer Zeit 37(1): 54-62

Deshuillers, P.L.; Santos, A.P.; do Nascimento, Níla.C.; Hampel, J.A.; Bergin, I.L.; Dyson, M.C.; Messick, J.B., 2014: Complete Genome Sequence of Mycoplasma ovis Strain Michigan, a Hemoplasma of Sheep with Two Distinct 16S rRNA Genes. We report the complete genome sequence of Mycoplasma ovis strain Michigan. Its single circular chromosome has 702,511 bp and contains 2 copies of the 16S rRNA gene, one corresponding to M. ovis and the other to "Candidatus Mycoplasma haemov...

Kay A., 1989: Monitoring of slow acting remission inducing sari drugs. British Journal of Rheumatology 28(3): 239-241

Mahfoud, F.; Lüscher, T.Felix., 2015: Renal denervation: symply trapped by complexity?. European Heart Journal 36(4): 199-202

Velluz, Leon, 1938: Inactivation de quelques toxines par la papaine. Compt Rend Soc Biol 128(16): 132-133

Patel, A.C.; Tyson, F.L.; Barrett, J.C.; Devereux, T.R., 1999: Hypermethylation of p16Ink4a in mouse lung tumor cell lines. Proceedings of the American Association for Cancer Research Annual Meeting 40: 441, March