geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Identification and molecular characterisation of hordoindolines from barley grain


, : Identification and molecular characterisation of hordoindolines from barley grain. Plant molecular biology 47(6): 785-794

Grain texture in barley is an important quality character as soft-textured cultivars have better malting quality. In wheat, texture is considered to be determined by the puroindolines, a group of basic hydrophobic proteins present on the surface of the starch granule. Hard wheats have been proposed to lack puroindoline a or to have mutant forms of puroindoline b which do not bind to the granule surface. Analysis of six barley cultivars (three soft-textured and three hard) showed that all contained proteins homologous to wheat puroindoline b, but PCR analysis failed to show any differences in amino acid sequences similar to those which have been proposed to determine textural differences in wheat. Southern blot analysis showed two hordoindoline b genes which were isolated and shown to encode proteins with 94% sequence identity. Expression of hordoindoline b mRNA occurred in the starchy endosperm and aleurone layer of the developing seed, but not in the embryo. Analysis of seven soft- and six hard-textured barley varieties showed that all contained hordoindoline a except two hard varieties (Sundance, Hart) which were subsequently shown to both lack hordoindoline a mRNA. It was therefore concluded that there is not a clear relationship between the presence of hordoindoline a and grain texture in barley.

US$19.90

DOI: 10.1023/a:1013691530675


Other references

Suzuki, T.; Ohashi, Y.; Oshika, T.; Goto, H.; Hirakata, A.; Fukushita, K.; Miyata, K., 2015: Outbreak of late-onset toxic anterior segment syndrome after implantation of one-piece intraocular lenses. To describe clinical findings associated with an outbreak of toxic anterior segment syndrome (TASS) after implantation of 1-piece intraocular lenses (IOLs; HOYA iSert(®) 251 and 255; HOYA Co Ltd, Tokyo, Japan) contaminated with aluminum. Multicen...

Amene, P.C., 1976: Intravenous fructose for acute alcoholism: a double blind study. To test the clinical efficacy of fructose intravenously for acute alcoholism, 20 acutely intoxicated patients were treated in a randomized, double blind fashion with 1000 cc of 10% fructose and 10% glucose over two hours. In the first hour of infu...

Howard, F.H.; Meriwether, W.A., 1952: Fat disease of the liver in infants on the Isthmus of Panama. Among infants examined post mortem at Gorgas Hospital between 1941 and 1951, severe fatty change in the liver was found in 48. None showed necrosis, and hepatic fibrosis was evident in only 2. In the pancreas histological changes were not consiste...

Hauser R.; McDonald D., 1992: Geology of the Nerco Con Mine, Yellowknife NWT. Exploration Overview

Luque, J.S.; Tyson, D.Martinez.; Markossian, T.; Lee, J-Hyun.; Turner, R.; Proctor, S.; Menard, J.; Meade, C.D., 2012: Increasing cervical cancer screening in a Hispanic migrant farmworker community through faith-based clinical outreach. Partnerships between academic medical centers and faith-based community organizations have been associated with increased screening rates in low-income minority women. We describe clinical outcomes of an outreach partnership between a cancer cente...

Mellini, E.; Gardenghi, G., 1994: Notes on anatomy and histology of the female reproductive system of Eucelatoria bryani Sabr. The female reproductive organs of Eucelatoria bryani Sabr., reared on the factitious host Galleria mellonella L., are studied and discussed in relation to its exceptional way of host attacking. Each ovary consists of just four-five ovarioles in wh...

Moskalenko V.N., 1992: Volcanic-sedimentary deposits in the Tyrrhenian abyssal basin. International Geology Review 34(2): 178-186

Dunn, D.T.; Newell, M.L.; Peckham, C.S.; Vanden Eijden, S., 1998: Routine vaccination and vaccine-preventable infections in children born to human immunodeficiency virus-infected mothers: European Collaborative Study. Information on vaccinations and vaccine-preventable infections collected in a prospective study of children born to human immunodeficiency virus (HIV)-infected mothers was analysed for reports of adverse reactions and to estimate the clinical effi...

Grifols J.; Grifols V.; Mas J., 1973: Quality control guide at the blood bank. Sangre (Saragossa) 18(3): 409

Jessup, J.M.; Menck, H.R.; Fremgen, A.; Winchester, D.P., 1997: Diagnosing colorectal carcinoma: clinical and molecular approaches. In summary, the ability to decrease the mortality of colorectal carcinoma is increasingly within the grasp of clinicians. With accurate family and personal history, it is possible to estimate the risk of colorectal cancer and initiate FOBT and col...