geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Identification and molecular characterization of a chitinase from the hard tick Haemaphysalis longicornis


, : Identification and molecular characterization of a chitinase from the hard tick Haemaphysalis longicornis. Journal of Biological Chemistry 278(10): 8556-8563

A cDNA encoding tick chitinase was cloned from a cDNA library of mRNA from Haemaphysalis longicornis eggs and designated as CHT1 cDNA. The CHT1 cDNA contains an open reading frame of 2790 bp that codes for 930 amino acid residues with a coding capacity of 104 kDa. The deduced amino acid sequence shows a 31% amino acid homology to Aedes aegypti chitinase and a multidomain structure containing one chitin binding peritrophin A domain and two glycosyl hydrolase family 18 chitin binding domains. The endogenous chitinase of H. longicornis was identified by a two-dimensional immunoblot analysis with mouse anti-rCHT1 serum and shown to have a molecular mass of 108 kDa with a pI of 5.0. A recombinant baculovirus AcMNPV[center dot]CHT1-expressed rCHT1 is glycosylated and able to degrade chitin. Chitin degradation was ablated by allosamidin in a dose-dependent manner. The optimal temperature and pH for activity of the purified chitinase were 45[degree]C and pH 5-7. The CHT1 cDNA has an ELR motif for chemokine-mediated angiogenesis and appears to be a chitinase of the chemokine family. Localization analysis using mouse anti-rCHT1 serum revealed that native chitinase is highly expressed in the epidermis and midgut of the tick. AcMNPV[center dot]CHT1 topically applied to H. longicornis ticks exhibited replication. This is the first report of insect baculovirus infection of ticks. The importance of AcMNPV[center dot]CHT1 as a novel bio-acaricide for tick control is discussed.

US$19.90

PMID: 12502707

DOI: 10.1074/jbc.M206831200


Other references

Zakirdzhaev, D.D.; Ashurov, B.M., 1983: Duplication method of increasing the strength of a deobliterated artery. Khirurgiia: 96-97

Gabler, E.; Jungnickel, G., 1968: M 632 Impuls herringbone milking parlour with automatic milking. Features of the new M 632 milking parlour, which can accommodate up to 35 cows, include easily dismantled piping, self-cleaning glass milk recorder, controlled dispensing of concentrates (to individual cows or groups), programmed milking (automati...

Melzer, R.; Theißen, Günter., 2016: The significance of developmental robustness for species diversity. The origin of new species and of new forms is one of the fundamental characteristics of evolution. However, the mechanisms that govern the diversity and disparity of lineages remain poorly understood. Particularly unclear are the reasons why some...

Chen, Z.W.; Liu, G.; Lu, X.F.; Liu, L.; Zhang, X.; Lau, T.C.K.; Tsui, S.K.W.; Kang, Y.X.; Zheng, P.R.; Zheng, B.J., 2016: Characterisation of novel anti-HIV/tuberculosis natural product analogues. Hong Kong Medical Journal 21 Suppl 7: S14-S17

Johnson A.M.; Sorenson H.O., 1976: Engadine dolostone of Michigan's eastern Upper Peninsula; geology and resource evaluation. Proceedings and Abstracts - Institute on Lake Superior Geology, Annual Meeting 22): 33-34

Tagliafierro G.; Faraldi G.; Bandelloni R., 1985: Distribution histochemistry and ultrastructure of somatostatin like immunoreactive cells in the gastroenteric tract of the cartilaginous fish scyliorhinus stellaris. Somatostatin-like immunoreactive cells of an open type have been identified in the digestive tract of the cartilaginous fish Scyliorhinus stellaris (L.) by the use of immunocytochemical techniques. In the stomach these cells are numerous both in t...

Rainey, M.C.; Zamboni, W.A.; Stephenson, L.L., 2001: The effect of hyperbaric oxygen on no concentrations in skeletal muscle undergoing ischemia-reperfusion injury. Journal of Investigative Medicine 49(1): 82A, January

Kölbel, F., 1969: Symposium on drugs and on the metabolism of the cardiac and skeletal muscle. Casopis Lekaru Ceskych 108(47): 1419-1420

Huang, Y-Lan.; Peng, C-Chih.; Chiu, N-Chang.; Lee, K-Sheng.; Hung, H-Yang.; Kao, H-An.; Hsu, C-Hsin.; Chang, J-Hsin.; Huang, F-Yuan., 2009: Bacterial tracheitis in pediatrics: 12 year experience at a medical center in Taiwan. Bacterial tracheitis may cause life-threatening airway obstruction. Records of patients admitted to the pediatric wards of Mackay Memorial Hospital between 1994 and 2005 with a diagnosis of bacterial tracheitis made on bronchoscopic visualization...

Goussetis, D.J.; Gounaris, E.; Wu, E.J.; Vakana, E.; Sharma, B.; Bogyo, M.; Altman, J.K.; Platanias, L.C., 2013: Autophagic degradation of the BCR-ABL oncoprotein and generation of antileukemic responses by arsenic trioxide. We provide evidence that arsenic trioxide (As(2)O(3)) targets the BCR-ABL oncoprotein via a novel mechanism involving p62/SQSTM1-mediated localization of the oncoprotein to the autolysosomes and subsequent degradation mediated by the protease cath...