geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Identification and mutagenicity of dinitrotoluene metabolites produced by strain Pseudomonas putida OU83


, : Identification and mutagenicity of dinitrotoluene metabolites produced by strain Pseudomonas putida OU83. Pesticide Biochemistry and Physiology 73(3): 131-139

Pseudomonas putida OU83 was isolated from soil contaminated with substituted aromatics. Resting cells of this bacterium incubated with dinitrotoluenes (DNTs) produced potentially biohazardous biotransformation products. The GC-MS analysis of the metabolites revealed fragmentation pattern of molecular ions at 152, 135, 107, and 77 amu, suggesting formation of methylnitroanilines. The identification of the metabolites was confirmed by the NMR spectral analysis. These results showed that 2,4-DNT, 3,4-DNT, and 2,6-DNT were transformed to 4-methyl-3-nitroaniline, 4-methyl-2-nitroaniline, and 2-methyl-3-nitroaniline, respectively. Relative biotransformations of 2,4-DNT, 3,4-DNT, and 2,6-DNT were to be 98%, 75%, and 40%, respectively in 48 h. These results indicate that strain OU83 enzymes catalyze nitro group of the DNT to an amino group and can be used for commercial production of methylnitroanilines, an intermediate for dye manufacturing. The mutagenicity of the metabolites of DNTs was increased, as tested by using Salmonella typhimurium strains TA98 in presence but not in the absence of arochlor 1254-induced (S9) rat liver mix. These findings suggest that the metabolites of DNTs produced by P. putida OU83 are promutagens.

US$19.90

DOI: 10.1016/s0048-3575(02)00102-5


Other references

Quay, WB., 1984: Winter tissue changes and regulatory mechanisms in nonhibernating small mammals: a survey and evaluation of adaptive and non-adaptive features. Carnegie Museum of Natural History Special Publication, 149-163 No. 10

Hardy, Re, 1980: The adolescent: rehabilitation medicine and social problems. Medical and sociological principles of adolescent care edited by George M Bright: 126

Uzelac-Keserović, B.; Spasić, P.; Bojanić, N.; Dimitrijević, J.; Lako, B.; Lepsanović, Z.; Kuljić-Kapulica, N.; Vasić, D.; Apostolov, K., 1999: Isolation of a coronavirus from kidney biopsies of endemic Balkan nephropathy patients. Endemic Balkan nephropathy (EBN) is a kidney disease of unknown etiology limited to Bulgaria, Rumania and former Yugoslavia. Primary kidney tissue cultures were established as explants from tissue obtained at operations from 5 EBN patients with ur...

Watkins, C.; Knight, E., 1959: Undergraduate psychiatric education as reflected in final examinations. American Journal of Psychiatry 116(1): 55-58

Brebner, K.; Froestl, W.; Roberts, D.C.S., 2002: The GABA(B) antagonist CGP56433A attenuates the effect of baclofen on cocaine but not heroin self-administration in the rat. Rationale: Several reports have demonstrated that the gamma-aminobutyric acid (GABA)B agonist baclofen attenuates the reinforcing effects of cocaine in rats, and recent evidence indicates that it might have a similar effect on heroin self-administ...

Shaldon S.; Chevallet M.; Mion C., 1976: Dialysis associated auto antibodies. Kidney International 10(2): 199

Meinzer Oscar Edward, 1934: Progress in the control of artesian-water supply. Engineering News-Record 113(6): 167-169

Jones, M.K.; Warner, E.B.; Oliver, J.D., 2008: csrA inhibits the formation of biofilms by Vibrio vulnificus. Polymerase chain reaction (PCR) screening of the shellfish-borne pathogen <i>Vibrio vulnificus</i> revealed <i>csrA</i>-negative strains, and these strains formed increased biofilm compared to <i>csrA</i>-positi...

National Shellfisheries Association, 2000: Technical Papers Presented at the 92nd Annual Meeting National Shellfisheries Association. Journal of Shellfish Research 19(1): 597-668, June

Blanc P.L.; Colin J.P., 1974: First results on the Maestrichtian ostracods of the Aquitaine Basin, France. Comptes Rendus Hebdomadaires des Seances de l'Academie des Sciences, Serie D: Sciences Naturelles 278(4): 425-428