geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Identification and phylogenetic analysis of a glucose transporter gene family from the human pathogenic yeast Candida albicans


, : Identification and phylogenetic analysis of a glucose transporter gene family from the human pathogenic yeast Candida albicans. Journal of Molecular Evolution 55(3): 336-346

We have identified a large family of glucose transporter genes (HGT1 to HGT20) from the human pathogenic yeast Candida albicans by screening of genomic sequences, reverse-transcription PCR assays, and phylogenetic analyses. The putative glucose transporter ORF sequences share among themselves 10-93% pairwise sequence identity and, in comparative analyses of predicted amino acid sequences, exhibit similarities to human and yeast transporters of the major facilitator superfamily (MFS): the predicted 12-transmembrane domains and sugar transporter signatures align closely to those of HXT transporters of Saccharomyces cerevisiae and GLUT transporters of humans, with amino acid residues at certain positions highly conserved throughout the families. Reverse-transcription PCR analyses demonstrated that the majority of the glucose transporters was transcribed in culture medium containing 2% glucose, while several were transcribed in the presence of low (0.2%) and/or high (5%) concentrations of glucose. Phylogenetic analyses revealed that there were three distinct clades of 20 HGT genes, which might represent three possible subfamilies. Additionally, HGT18 and HGT20 show a high overall sequence identity to the human GLUTs, indicating a possible functional convergence. We conclude that glucose transporters in C. albicans comprise a family of 20 known members, with variable transcription in response to glucose concentration.

US$19.90

PMID: 12187386

DOI: 10.1007/s00239-002-2330-4


Other references

Barnett, A.G.; Herbert, D.L.; Campbell, M.; Daly, N.; Roberts, J.A.; Mudge, A.; Graves, N., 2016: Streamlined research funding using short proposals and accelerated peer review: an observational study. Despite the widely recognised importance of sustainable health care systems, health services research remains generally underfunded in Australia. The Australian Centre for Health Services Innovation (AusHSI) is funding health services research in...

Perlman, M.D.; Wu, L., 2004: A note on one-sided tests with multiple endpoints. Testing problems with multivariate one-sided alternative hypotheses are common in clinical trials with multiple endpoints. In the case of comparing two treatments, treatment 1 is often preferred if it is superior for at least one of the endpoints...

Anonymous, 1993: Agriculture Department scientists work to protect citrus from tropical pest. Genetic engineering news, 13(6): 14

Itoh, N.; Kunihara, M.; Kushida, H.; Mcwhorter, W.W.; Nomura, S.; Ozawa, K.; Taniguchi, M.; Tsuzuki, K., 1997: Tropolone derivatives and pharmaceutical composition thereof for preventing and treating ischemic diseases. Official Gazette of the United States Patent & Trademark Office Patents 1194(2): 1286-1287, Jan 14

Crow, Jf, 1968: The ultraselfish gene. Genetics 118(3): 389-391

Pelizzo, M.R.; Toniato, A.; Grigoletto, R.; Piotto, A.; Bernante, P., 1995: Post-thyroidectomy hypocalcemia and ligation of the inferior thyroid artery trunk. The effect of bilateral truncal ligation of the inferior thyroid artery on parathyroid function is prospectively analyzed in two homogeneous groups of 10 patients treated by total thyroidectomy, with (G1) and without (G2) truncal ligation, compari...

Pigorini B.; Soggetti F.; Veniale F., 1968: Petrogenetical significance of the accessory zircon in the granites from Baveno, M. Orfano and Alzo (north Italy). International Geological Congress, Abstracts--Congres Geologique Internationale, Resumes 23: 349

Mukunyadzi, P.; Johnson, M.; Wyble, J.G.; Scott, M., 2002: Diagnosis of histoplasmosis in urine cytology: reactive urothelial changes, a diagnostic pitfall. Case report and literature review of urinary tract infections. Histoplasmosis not uncommonly causes systemic infection, particularly in immunocompromised patients. In systemic infection, the urinary tract is often involved, although the diagnosis of histoplasmosis in urine cytologic specimens has never been r...

Hengl, T.; Sierdsema, H.; Radovic, A.; Dilo, A., 2009: Spatial prediction of species distributions from occurrence-only records combining point pattern analysis, ENFA and regression-kriging. A computational framework to map species' distributions (realized density) using occurrence-only data and environmental predictors is presented and illustrated using a textbook example and two case studies: distribution of root vole (Microtes...

Lech, M.M.; Swiatek, E.J., 2001: Practices of prescribing oral contraceptives in Poland. We developed and performed a survey on practices of prescribing oral contraceptives in Poland. The survey was carried out in Warsaw, the capital city of Poland and one of the most important areas of the country (approximately 4 million inhabitants...