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Identification by PCR of Fusarium culmorum strains producing large and small amounts of deoxynivalenol


, : Identification by PCR of Fusarium culmorum strains producing large and small amounts of deoxynivalenol. Applied and environmental microbiology 68(11): 5472-5479

Thirty deoxynivalenol-producing F. culmorum strains, isolated from wheat grains, were incubated in vitro and analyzed for trichothecene production. Seventeen strains produced more than 1 ppm of deoxynivalenol and acetyldeoxynivalenol and were considered high-deoxynivalenol-producing strains, whereas 13 F. culmorum strains produced less than 0.07 ppm of trichothecenes and were considered low-deoxynivalenol-producing strains. For all strains, a 550-base portion of the trichodiene synthase gene (tri5) was amplified and sequenced. According to the tri5 data, the F. culmorum strains tested clustered into two groups that correlated with in vitro deoxynivalenol production. For three high-producing and three low-producing F. culmorum strains, the tri5-tri6 intergenic region was then sequenced, which confirmed the two separate clusters within the F. culmorum strains. According to the tri5-tri6 sequence data, specific PCR primers were designed to allow differentiation of high-producing from low-producing F. culmorum strains.

US$19.90

PMID: 12406740

DOI: 10.1128/aem.68.11.5472-5479.2002


Other references

Ossiannilsson, F., 1943: Studies on the insect fauna of the Swedish Potato field and its importance in the spread of virus diseases. I. Hemiptera, occurrence and distribution. Full particulars are given of the characteristics, biology, and geographical distribution in Sweden and elsewhere of Myzus persicae, M. pseudosolani, Macrosiphum gei, and Aphis rhamni, vectors of potato virus diseases, and of a number of other Hem...

Smith, A.C.; Choufani, S.; Ferreira, J.C.; Weksberg, R., 2007: Growth regulation, imprinted genes, and chromosome 11p15.5. Genomic imprinting refers to parent-of-origin-specific gene expression. Human chromosome band 11p15.5 houses a large cluster of genes that are imprinted. Dysregulation of this gene cluster is associated with the overgrowth and tumor predisposition...

Anonymous, 1978: Roster. Mississippi Dental Association. Mississippi Dental Association Journal 35(4): 20-26

Salazar, A.L., 1946: The interstitial lymph of the smooth muscle tissue and the problem of the manchons pellucides. By the tannin-iron method, lymph spaces can be demonstrated in every smooth muscle, particularly those of the blood vessels. They are in communication with those of the connective tissue and form a circulatory system. The network of "Gitterfa...

Anonymous, 1968: Department of Transportation, Federal Aviation Administration, Office of Aviation Medicine. Questions and answers of interest to aviation medical examiners. Aerospace Medicine 39(4): 427-427

Formicola, G.; Iannaccone, G.; Monaco, T., 1981: Fertility and cryptorchism. Preliminary considerations. Minerva Urologica 33(3): 175-180

Garcia-Carvalho, E.; Criscuolo-Urbinati, E., 2005: Crescimento, desenvolvimento gonadal e composicao muscular de matrinxas (Brycon cephalus) submetidos a restricao alimentar e realimentacao durante um ano. This work determined the effect of food restriction muscle composition of matrinxa, Brycon cephalus, throughout a year (Jan 1998 to Jan 1999). A total of 135 fish, males and females, were split out into two groups. The control group received ratio...

Goncalves P.D.S.; Rosetti A.G.; Valois A.C.C.; Viegas J.I.D., 1984: Genetic and phenotypic correlations between some quantitative traits in juvenile clonal rubber trees hevea spp. A study was designed to determine the genetic correlation (rG) and phenotypic correlation (rF) among 10 characters of 14 rubber tree juvenile clones. The characters were: yield of dry rubber per tapping by the early Mendes test (P), plant height (...

Ozbey, O.; Ozcelik, M., 2004: The effect of high environmental temperature on growth performance of Japanese quails with different body weights. The aim of this study was to determine the effect of high environmental temperature on liveweight, feed consumption, feed efficiency, survival and some carcass features of Japanese quails with different body weights. During the research, a total o...

Fininsa, C.; Tefera, T., 2002: Inoculum sources of bean anthracnose and their effect on epidemics and yield. In a field experiment conducted during 1997 and 1998 in Ethiopia, inoculum sources of Colletotrichum lindemuthianum significantly influenced anthracnose incidence and severity levels during the early growth of common bean (Phaseolus vulgaris). Hig...