geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Identification in traditional herbal medications and confirmation by synthesis of factors that inhibit cholera toxin-induced fluid accumulation


, : Identification in traditional herbal medications and confirmation by synthesis of factors that inhibit cholera toxin-induced fluid accumulation. Proceedings of the National Academy of Sciences of the United States of America 99(5): 3042-3046

Kampo formulations are traditional herbal medications used in China and Japan for many centuries to treat diarrheal diseases such as cholera. Our studies were undertaken to identify and verify by chemical synthesis the active components that inhibited cholera toxin (CT), the virulence factor secreted by Vibrio cholerae, the causative agent of cholera. The Kampo formulation, Daio-kanzo-to, inhibited CT activities (i.e., ADP-ribosylation, Chinese hamster ovary cell elongation); in Daio-kanzo-to, Daio (Rhei rhizoma) was responsible for this effect. Among several components purified from Daio extract, rhubarb galloyl-tannin, a compound characterized by a polygallate structure, was the most effective. To define the active component, gallate analogues similar to rhubarb galloyl-tannin were synthesized. These gallate compounds inhibited all CT activities including ADP-ribosylation, elongation of Chinese hamster ovary cells, and importantly, fluid accumulation in ileal loops. Thus, Kampo formulations or their gallate components might be effective adjunctive therapy with oral rehydration solution for the severe diarrhea of cholera.

US$19.90

PMID: 11854470

DOI: 10.1073/pnas.052709499


Other references

Ericsson, U.B.; Bjerre, I.; Forsgren, M.; Ivarsson, S.A., 1985: Thyroglobulin and thyroid hormones in patients on long-term treatment with phenytoin, carbamazepine, and valproic acid. Serum concentrations of total triiodothyronine (T3) and thyroxine (T4) as well as serum thyroid-stimulating hormone (TSH) and thyroglobulin (Tg) were measured in 24 patients with epilepsy taking anticonvulsants (either phenytoin, carbamazepine, or...

Anonymous, 2012: New CoC standards focus on patient-centered care. Ons Connect 26(11): 21-21

Weech, M.; Quash, M.; Walters, E., 2003: Characterization of the mouse olfactory glutathione S-transferases during the acute phase response. The acute phase response (APR) has been shown to alter expression and activity of biotransformation enzymes, such as the phase I cytochromes p450 and phase II glutathione S-transferases (GSTs). The cytochromes p450 and GSTs are expressed abundantl...

Nishimura, M.; Ieshima, A.; Takashima, S.; Takeshita, K., 1986: Focal vulnerability of developing brain: CT studies on disproportionally dilated lateral ventricles. Cranial computed tomography (CT) of 108 cases with dilated lateral ventricles was reviewed to elucidate the relationship between focal vulnerability of developing brain and disproportional dilatation of lateral ventricles. CT findings of 108 cases...

Chocair, P.R.; Duley, J.A.; Sabbaga, E.; Arap, S.; Simmonds, H.A.; Cameron, J.S., 1993: Fast and slow methylators: do racial differences influence risk of allograft rejection?. A catabolic route for azathioprine involving methylation by thiopurine methyltransferase has been directly implicated in the drug's immunosuppressive efficacy. Since ethnic differences in thiopurine methyltransferase activity have been report...

Camargo L.D.S.; Alves S.; Scaranari H.; Abramides E., 1968: New strawberry d cultivar for the paulista plateau area conditions. Bragantia 27(10): 117-132

Kema, G.H.; van Silfhout, C.H., 1997: Genetic Variation for Virulence and Resistance in the Wheat-Mycosphaerella graminicola Pathosystem III. Comparative Seedling and Adult Plant Experiments. ABSTRACT Fourteen Dutch Mycosphaerella graminicola isolates were studied for their virulence to 22 wheat cultivars in the seedling stage in an experiment set up as a completely randomized block design with three repetitions. Isolate x cultivar int...

Moreno, E.; Hidalgo, M.; Hebrero, J.; Ruíz, L.P.; Jelavic, D.; Solozabal, J.; Solis, J.; Ramirez, J.; Arias, E.; Casanova, A., 1976: Coronary-caval anastomosis in the treatment of portal hypertension. Revista Clinica Espanola 141(6): 565-572

Zhang, C.Y.; Yamasaki, R.; Mitsuhashi, S.; Takahashi, H.; Bando, H.; Saito, S., 1991: Radioimmunoassay of growth hormone-releasing hormone (GHRH) with a polyclonal antibody against synthetic GHRH(1-29)-Gly4-Cys-NH2: method and clinical studies. A radioimmunoassay (RIA) for growth hormone-releasing hormone (GHRH) using a polyclonal antibody against synthetic GHRH(1-29)-Gly4-Cys-NH2 has been developed. The antiserum (RBM105) showed full cross-reactivity with GHRH-(1-44)NH2, GHRH-(1-40)OH,...

Visentini, E.; Bondavalli, C.; Pegoraro, C.; Schiavon, L.; Dall'Oglio, B.; Parma, A., 1990: Ovarian remnant syndrome: a case report. A case is reported of ureteral obstruction due to retained corpus luteum. The patient had previously undergone a hysterectomy and salpingo-ovariectomy with incomplete removal of ovarian tissue on both sides. The ureteral obstruction was treated by...