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Identification of ALS inhibitor-resistant Amaranthus biotypes using polymerase chain reaction amplification of specific alleles

, : Identification of ALS inhibitor-resistant Amaranthus biotypes using polymerase chain reaction amplification of specific alleles. Weed Research Oxford 42(4): 280-286

Polymerase chain reaction (PCR) amplification of specific alleles (PASA) was adapted as a molecular marker-based method for the rapid detection of point mutations in Amaranthus retroflexus and A. rudis leading to acetolactate synthase (ALS) inhibitor resistance. Two pairs of primers were designed for the specific amplification of alleles of the ALS gene of susceptible and resistant biotypes.


DOI: 10.1046/j.1365-3180.2002.00285.x

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