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Identification of Cryptosporidium spp. oocysts in fecal smears stained with Heidenhain's iron hematoxylin

, : Identification of Cryptosporidium spp. oocysts in fecal smears stained with Heidenhain's iron hematoxylin. Revista do Instituto de Medicina Tropical de Sao Paulo 43(6): 341-342

There is no paucity of methods for diagnosing Cryptosporidium spp. infection. The merits of immunoassays notwithstanding, microscopic identification of Cryptosporidium spp. oocysts in fecal samples remains an important diagnostic procedure. It owes the persistence of its use to such characteristics as dispensing with expensive equipment and kits, requiring only basic laboratory facilities, and having a low probability of false positive results when permanent slides are prepared, which can be re-examined in case of doubt. Cryptosporidium spp. oocysts can be readily identified in fecal smears prepared according to a regressive iron hematoxylin staining technique. The number of steps and their duration, as well as costs, were reduced to a minimum without loss of image quality and permanence of the preparations.


Other references

Sterner, R.; Boffa, L.C.; Vidali, G., 1978: Comparative structural analysis of high mobility group proteins from a variety of sources. Evidence for a high mobility group protein unique to avian erythrocyte nuclei. High mobility group (HMG) proteins were isolated from a variety of sources by extraction of purified nuclei with 0.35 M NaCl followed by selective trichloroacetic acid precipitation. The protein sources included thymus, kidney and liver from calf,...

Pylatuik, J.D.; Bonham Smith, P.C.; Davis, A.R., 1997: How virile is SUPERMAN ?. American Journal of Botany 84(6 SUPPL ): 52

Kramer, M., 2005: Long-term use of hypnotic agents in the treatment of chronic insomnia. Psychiatric Services 56(6): 752; Author Reply 752-3

Flegel, H., 1965: Further studies on the role of staphylococci in eczema. Der Hautarzt; Zeitschrift für Dermatologie, Venerologie, und Verwandte Gebiete 16(9): 406-407

Opas, M., 1997: Measurement of intracellular pH and pCa with a confocal microscope. In the late 1980s, the field of biological confocal microscopy exploded. So did traffic on the Internet. Considering the ongoing interest in the role of intracellular pH and pCa in all aspects of cell physiology, it is not surprising that the most...

Hauge, L.Johan.; Kornstad, T.; Nes, R.Bang.; Kristensen, P.; Irgens, L.M.; Eskedal, L.T.; Landolt, M.A.; Vollrath, M.E., 2014: The impact of a child's special health care needs on maternal work participation during early motherhood. Many women temporarily reduce work hours or stop working when caring for small children. However, mothers of children with special health care needs may face particular challenges balancing childrearing responsibilities and employment demands. Thi...

Meyboom, R.H., 1991: Influence of oral contraceptives on body temperature. Bmj 302(6783): 1025-1025

Jong, J.D.; Garretsen, F., 1985: Genetic analysis of cut flower longevity in Gerbera. The vase life duration and stem stiffness of 12 parent clones and 77 progenies of Gerbera jamesonii were studied both in summer and in winter. In winter, vase life was shorter and the stems were weak. Few exceptions from this general pattern was d...

Hunt, T.W.; Carroll, R.C.; Peralta, E.G., 1993: G-alpha-i3 mediates pertussis toxin-sensitive activation of phospholipase C by the M2 muscarinic acetylcholine receptor in CHO cells. Journal of Cellular Biochemistry Supplement 0(17 PART D): 257

Smith, Richard, 2009: Mr Popes pots and the rapid smelting of Lake District copper and lead ores, 1579-83. British Mining 88