geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Identification of Pantoea stewartii subsp. stewartii by PCR and strain differentiation by PFGE


, : Identification of Pantoea stewartii subsp. stewartii by PCR and strain differentiation by PFGE. Plant Disease 86(3): 304-311, March

Stewart's bacterial wilt and leaf blight of sweet corn and maize is caused by Pantoea stewartii subsp. stewartii. This bacterium can be seed transmitted at a low frequency, so it is subject to quarantine restrictions by many countries. To develop a polymerase chain reaction assay for the identification of this pathogen from field samples and for use in seed health tests, four primer pairs were tested. These were selected from the sequences of hrpS, cpsDE, and the 16S rRNA intergenic transcribed spacer (ITS) region. Under optimal reaction conditions, about 20 and 200 cells of P. stewartii could be detected in pure cultures and leaf lesions, respectively. Other plant-associated enteric bacteria (e.g., P. agglomerans pv. herbicola, P. ananas, Erwinia amylovora, and E. carotovora) either did not produce amplicons or they were not the correct size for P. stewartii. To test further for possible false positives, 29 yellow-pigmented bacteria, mainly other Pantoea spp., were isolated from lesions on old corn leaves and assayed with the ITS primer sets. Except for weak, variable reactions with three P. ananas strains, the bacteria did not test positive. Pulsed field gel electrophoresis (PFGE) was evaluated as an additional test to confirm the identity of P. stewartii. After digestion with SpeI and XbaI, P. stewartii strains could be easily distinguished from related Erwinia and Pantoea spp. and each other.

US$19.90

DOI: 10.1094/pdis.2002.86.3.304


Other references

Hoffmann, O.; Angelov, N.; Gallez, F.; Jung, R.E.; Weber, F.E., 2008: The zirconia implant-bone interface: a preliminary histologic evaluation in rabbits. Zirconia ceramics, a biocompatible material with favorable mechanical properties, has been suggested for use in the manufacture of dental implants instead of the commonly used titanium. Not much data exist on the early healing response around zirc...

Tiwari, P.K.; Chauhan, M., 1994: Salivary gland polytene chromosomes of Chironomus circumdatus Kiefer and Chironomus javanus Kiefer. Agrawal, O P [Editor] Perspectives in entomological research 211-226

Lahloub M.F.; Goda M.; Affifi M.S.; Sticher O., 1989: Iridoid glucosides from veronica anagallis aquatica var anagalloides. Planta Medica 55(7): 627

Schierenbeck, K.A.; Mack, R.N.; Sharitz, R.R., 1994: Effects of herbivory on growth and biomass allocation in native and introduced species of Lonicera. Growth and biomass allocation patterns of an invasive vine, Lonicera japonica, and its native congener, L. sempervirens, were compared for 3 herbivory treatments in the southeastern U.S. The objectives of the study were to determine the comparativ...

Beilby, M.J.; Coster, H.G.L., 1979: The action potential in chara corallina 3. the hodgkin huxley parameters for the plasmalemma. An analysis, based on the Hodgkin-Huxley (H-H) equations is given of excitation processes in the plasmalemma of cells of C. corallina. Voltage clamp data for the plasmalemma show the presence of 2 activation and 2 inactivation processes and both o...

Schuler, S.R.; Hashemi, S.M., 1995: Family planning outreach and credit programs in rural Bangladesh. The paper is the result of recent research in rural Bangladesh which suggests that programmes that draw women out of their homes and reduce their dependence on men are contributing to greater use of contraception. One such programme, Grameen Bank,...

Sankardas, M.A.; McEniery, P.T.; Aroney, C.N.; Bett, J.H., 1996: Elective implantation of intracoronary stents without intravascular ultrasound guidance or subsequent warfarin. Two hundred forty-three stents (203 Palmaz-Schatz, 40 Glanturco-Roubin) were electively Implanted in 188 lesions in 168 patients (mean age 58 +/- 10 years, 77% males) using angiographic but not ultrasound guidance. Patients were treated subsequent...

Kipreos, E.T., 2016: Implications of an absolute simultaneity theory for cosmology and universe acceleration. An alternate Lorentz transformation, Absolute Lorentz Transformation (ALT), has similar kinematics to special relativity yet maintains absolute simultaneity in the context of a preferred reference frame. In this study, it is shown that ALT is comp...

Broeze, R.J.; Dougherty, J.P.; Pichon, J.; Jayaram, B.M.; Lengyel, P., 1981: Studies with pure mouse Ehrlich ascites tumor interferons alpha and beta: patterns of induction of (2'-5') (A)n synthetase and of a double-stranded RNA-dependent protein kinase in mouse cells and human cells. The N-terminal sequences of mouse Ehrlich ascites tumor cell IFN beta (35,000-40,000 daltons) and IFN alpha (20,000 daltons) differ in 18 out of 20 positions. Furthermore, these two IFN species show little immunological cross reactivity. We treate...

Wimmershoff, M.B.; Hohenleutner, U.; Landthaler, M., 2000: Isomorphic phenomenon: adverse effect after epilation with the long-pulsed ruby laser. Archives of Dermatology 136(12): 1570-1571