geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Identification of Plasmodium falciparum, P. vivax, P. ovale and P. malariae and detection of mixed infection in patients with imported malaria in Italy


, : Identification of Plasmodium falciparum, P. vivax, P. ovale and P. malariae and detection of mixed infection in patients with imported malaria in Italy. New Microbiologica 26(1): 91-100

The species-specific nested-PCR previously described by Snounou and others for detecting the four parasite species that cause human malaria is evaluated in the current study testing 230 blood samples. The results are compared with those obtained by microscopy and, for 101 samples out of 230, with those previously obtained by a genus-specific PCR based method (pg-PCR) followed by species-specific Southern-blot hybridization. All blood specimens were obtained from patients (127 foreigners and 103 Italians) with a suspect clinical diagnosis of imported malaria in Italy: 76 were positive by microscopy and 83 were positive by nested-PCR. The last method also revealed 10 double infections (8 foreigners and 2 Italians) which were not identified by microscopy or by pg-PCR with species-specific Southern-blot hybridization. Fifty-four out of 83 positive samples tested by nested-PCR were submitted to genomic sequence analysis, which confirmed the presence of DNA region portion encoding the 18S rRNA corresponding to the Plasmodium species identified by nested-PCR. These results demonstrate that the nested-PCR assay surpasses microscopy and pg-PCR with species-specific Southern-blot hybridization, both in sensitivity and in diagnostic accuracy. Moreover, it is quicker because it requires no further blotting or hybridization of PCR amplification products. This method also offers a clear advantage in the detection of mixed infections, which is important not only for successful medical treatment but also for the study of malaria epidemiology. Finally, our study also highlights the value of genomic sequence analysis for validating PCR results.

US$29.90

PMID: 12578316


Other references

Bara J., 1980: Immuno histological study of the cell differentiation in human gastro intestinal cancers. Using immunofluorescence, well differentiated mucus cells which are specialized in the synthesis of M antigens were found in the normal adult gastrointestinal tract. Mucus cells of surface epithelium show M1 antigens, those of gastric and intestin...

Potapov, A.I., 1970: Productivity and inheritance of meat-fat and fat types of young Livny pigs. For meat-fat and fat types resp. at 12.18 mth of age, live weight averaged 164.7 and 165.8 kg, body length 147.6 and 143.6 cm, and chest circumference 130.9 and 137.9 cm; for animals of the 2 types resp. aged 33 mth or more the corresponding measu...

Bocchiaro, C.M.; Saywell, S.A.; Feldman, J.L., 2003: Dynamic modulation of inspiratory drive currents by protein kinase A and protein phosphatases in functionally active motoneurons. Plasticity underlying adaptive, long-term changes in breathing behavior is hypothesized to be attributable to the modulation of respiratory motoneurons by intracellular second-messenger cascades. In quiescent preparations, protein kinases, includi...

Kerr, D.A.; Larsen, T.; Cook, S.H.; Fannjiang, Y-Ru.; Choi, E.; Griffin, D.E.; Hardwick, J.Marie.; Irani, D.N., 2002: BCL-2 and BAX protect adult mice from lethal Sindbis virus infection but do not protect spinal cord motor neurons or prevent paralysis. Cellular proteins that regulate apoptotic cell death can modulate the outcome of Sindbis virus (SV) encephalitis in mice. Both endogenous and overexpressed BCL-2 and BAX proteins protect newborn mice from fatal SV infection by blocking apoptosis i...

Daguzan, J., 1981: Contribution a l'elevage de l'escargot petit-gris: Helix aspersa Muller (mollusque gasteropode pulomone stylommatophore). 1. Reproduction et eclosion des jeunes, en batiment et en conditions thermohygrometriques controlees. The number of edible snails is decreasing in France and in Europe, and it is now necessary to develop snail raising or heliciculture. A production program involving the Petit-gris snail (H. aspersa Mueller) was started at the National Institute fo...

Cunningham, C.; Cho, E.; Shaffer, M.L.; Bradford, M.C.; Palermo, T., 2016: Screening for Psychosocial and Family Risk in Pediatric Gastrointestinal Disorders. Screening for psychosocial comorbidity is recommended for pediatric patients presenting at an initial gastroenterology (GI) outpatient consultation. We developed and evaluated the psychometric properties of the GI Screener to address the need for...

Heffner; Cox, 1989: Comment on "Observation of magnetic ordering in superconducting La2-xSrxCuO4 by muon spin rotation". Physical Review Letters 63(22): 2538-2538

Alcamo, A.M.; Davids, A.R.; Way, D.P.; Lynn, D.Joanne.; Vandre, D.D., 2010: The impact of a peer-designed and -led USMLE Step 1 review course: improvement in preparation and scores. Medical students use several strategies for United States Medical Licensing Examination (USMLE) Step 1 preparation. At Ohio State University College of Medicine, a yearlong, peer-designed and -led Step 1 review course is a new option for our secon...

Usandizaga, M., 1968: Extraction of the fetal head by pressure from the perineum. Special mention of facial presentation. Acta Obstetrica Y Ginecologica Hispano-Lusitana 16(6): 465-473

Morin Denis, 1994: Typology of exploitation systems of sedimentary iron ores in a karstic environment; the example of Franche-Comte in the XVIth to XIXth centuries. Reunion Annuelle des Sciences de la Terre 15: 149