geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Identification of Pythium oligandrum using species-specific ITS rDNA PCR oligonucleotides


, : Identification of Pythium oligandrum using species-specific ITS rDNA PCR oligonucleotides. Mycological Research 107(7): 790-796

Pythium oligandrum is a parasite of cultivated Agaricus bisporus. Infection results in significant yield reductions and a disease referred to as 'black compost'. In this study, P. oligandrum isolates were isolated from New Zealand mushroom composts, and their ribosomal DNA internal transcribed spacer (ITS) regions were amplified using the polymerase chain reaction (PCR). ITS nucleotide sequences obtained from New Zealand P. oligandrum isolates were compared with those previously identified P. oligandrum isolates and 23 described Pythium species. Although New Zealand P. oligandrum isolates had high ITS nucleotide identity with internationally identified P. oligandrum, the order of nucleotides in some regions varied when compared with other Pythium species. These varied nucleotides within the ITS region were used to design PCR primers (P.OLIG.F1 and P.OLIG.R04) for the specific amplification of a 384-bp fragment from P. oligandrum DNA. P.OLIG.F1 and P.OLIG.R04 were used to identify a major source of P. oligandrum inoculation on a New Zealand mushroom farm. Application of this diagnostic test will assist farming strategies implemented to prevent future P. oligandrum outbreaks. Furthermore, results presented identify a need for species resolution between P. oligandrum and P. hydnosporum.

US$19.90

PMID: 12967205

DOI: 10.1017/s0953756203008104


Other references

Denney, W.L., 1932: The Treatment Of Distension Following Abdominal Operations In Children. Canadian Medical Association Journal 26(4): 430-431

Uzar, H.C.; Battersby, A.R.; Carpenter, T.A.; Leeper, F.J., 1987: Biosynthesis of porphyrins and related macrocycles part 28. development of a pulse labelling method to determine the c methylation sequence for vitamin b 12. The biosynthesis of vitamin B12 involves enzymic C-methylation of uroporphyrinogen-III at 8 sites. Earlier work had established that the first 3 methyl groups are introduced at positions 2, 7, and 20 in that order. Pulse-labelling experiments with...

Rooney, J.R., 1957: Paper electrophoresis of bovine serum. A system for the paper electrophoretic analysis of bovine serum proteins is described. A 5-hour run at 21 ma constant current, using a [mu] = 0.053 veronal buffer system is reccomended. The hanging strip cell and Whatman 3-mm filter paper were use...

Blazevich, A.J.; Cannavan, D.; Horne, S.; Coleman, D.R.; Aagaard, P., 2009: Changes in muscle force-length properties affect the early rise of force in vivo. Changes in contractile rate of force development (RFD), measured within a short time interval from contraction initiation, were measured after a period of strength training that led to increases in muscle fascicle length but no measurable change i...

Brücher, Börn.L.D.M.; Swisher, S.G.; Königsrainer, A.; Zieker, D.; Hartmann, Jörg.; Stein, H.; Kitagawa, Y.; Law, S.; Ajani, J.A., 2009: Response to preoperative therapy in upper gastrointestinal cancers. Annals of Surgical Oncology 16(4): 878-886

Raoul, J-Luc.; Gilabert, M.; Oziel-Taïeb, S.; Giovannini, M., 2013: Palliative treatment for hepatocellular carcinoma. Following the Barcelona staging system of hepatocellular carcinoma in 5 stages, palliative medical treatment have to be given to end-stage patients (best supportive care) and to advanced and intermediate stages. Chemoembolization is the standard o...

Skacel, M.; Siva, A.; Xu, B.; Tubbs, R.R., 2006: From array to array: confirmation of genomic gains and losses discovered by array-based comparative genomic hybridization utilizing fluorescence in situ hybridization on tissue microarrays. The combination of array-based comparative genomic hybridization (CGH) with fluorescence in situ hybridization utilizing custom-designed bacterial artificial chromosome (BAC) probes applied to tissue microarrays represents a powerful compendium o...

Tsiavou, A.; Degiannis, D.; Hatziagelaki, E.; Koniavitou, K.; Raptis, S., 2000: Intracellular IFN-gamma release in latent autoimmune diabetes of adults and in non-insulin dependent diabetes of adults. Diabetologia 43(Supplement 1): A108, August

Herger, P.; Dieckmann, L., 1985: Die Insekten fauna des Hochmoores Balmoos bei Hasle, Kanton Luzern. 22. Coleoptera (Kafer) 4: Curculionidae. Entomologische Berichte Luzern, 87-90 No. 13

Vilela, S.M. de O.; Pinheiro Junior, J.W.; Barbosa, R.A.; Medeiros, E.S. de; Mota, R.A., 2006: Outbreak of colisepticemia in commercial layers in chicken farms in Pernambuco. The objective of this study was to investigate the occurrence of various outbreaks of colisepticaemia in commercial layers from farms in Pernambuco, Brazil. The clinical symptoms shown were apathy, cyanotic comb and swollen head. Necropsy findings...