+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Identification of RAPD markers linked to the Uvf-1 gene conferring hypersensitive resistance against rust (Uromyces viciae-fabae) in Vicia faba L

, : Identification of RAPD markers linked to the Uvf-1 gene conferring hypersensitive resistance against rust (Uromyces viciae-fabae) in Vicia faba L. Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 107(2): 353-358

Bulk segregant analysis was used to identify random amplified polymorphic DNA (RAPD) markers linked to a gene determining hypersensitive resistance in Vicia faba line 2N52 against race 1 of the rust fungus Uromyces viciae-fabae. The monogenic nature of the resistance was determined by analyzing the F(2) population from a cross between resistant line 2N52 and susceptible line VF-176, and further confirmed in the F(2:3)-derived families. Linkage of the RAPD markers was confirmed by screening 55 F(2) plants segregating for resistance. Three RAPD markers (OPD13(736), OPL18(1032) and OPI20(900)) were mapped in coupling phase to the resistance gene for race 1 ( Uvf-1). No recombinants between OPI20(900) and Uvf-1 were detected. Two additional markers (OPP02(1172) and OPR07(930)) were linked to the gene in repulsion phase at a distance of 9.9 and 11.5 cM, respectively. The application of marker-assisted selection to develop new faba bean varieties with rust resistance genes is discussed.


PMID: 12698251

DOI: 10.1007/s00122-003-1254-8

Other references

Kogbe C.A., 1993: Mineral exploration techniques. Pages 43-45 1993

Smith, Maxwell., 1907: A new varietal form of Turbo petholatus, Linn. Bulletin of the Brooklyn Conchological Club Brooklyn N Y: 1 5-6

Gao, J.; Shi, Z.; Xu, L.; Yang, X.; Jia, Z.; Lü, S.; Feng, C.; Shang, J., 2013: Precipitation variability in Hulunbuir, northeastern China since 1829 AD reconstructed from tree-rings and its linkage with remote oceans. Precipitation is the main factor limiting the radial growth of <italic>Pinus sylvestris</italic> var. <italic>mongolica</italic> in the Hulunbuir region, northeastern China. We used tree-ring width data to reconstruct preci...

Musteikaite, L.; Kazlauskas, J.; Danilevicius, E., 1961: On the intestinal helminths of dogs and cats. Acta Parasitologica Lithuanica, 3: 17-20

Shlyapnikov, S.V.; Arutyunyan, A.A.; Kurochkin, S.N.; Memelova, L.V.; Nesterova, M.V.; Sashchenko, L.P.; Severin, E.S., 1976: Phosphorylation of lysine rich histones using histone kinase from pig brain. The phosphorylation of lysine-rich histones F1, F2a2 and F2b of calf thymus was investigated using homogeneous histone kinase from pig brain. 32P-labeled phosphopeptides from tryptic digests of corresponding histones were obtained. According to N-...

Brunner, J.F., 1995: Bioassay, tests of B thuringiensis products against overwintering and non-overwintering leaf-roller larvae, 1993. Burditt, A K , Jr Arthropod Management Tests 340-341

Murray Alison M., 2002: Lower pharyngeal jaw of a cichlid fish (Actinopterygii, Labroidei) from an early Oligocene site in the Fayum, Egypt. African fossil cichlids are known from deposits ranging in age from Eocene through to Quaternary. The Eocene cichlids, five species of Mahengechromis, are from deposits in Tanzania, dated at 45 My old (Murray, 2000). Fossil cichlids probably of Ol...

Kim, W.T.; Silverstone, A.; Yip, W.K.; Dong, J.G.; Yang, S.F., 1992: Induction of 1-aminocyclopropane-1-carboxylate synthase mRNA by auxin in mung bean hypocotyls and cultured apple shoots. Auxin is known to promote ethylene production in vegetative tissues by increasing the activity of 1-aminocyclopropane-l-carboxylate (ACC) synthase; therefore, we have studied the effect of auxins on ACC synthase mRNA expression. Total RNA was isol...

Siegle, R.L.; Lieberman, P., 1978: A review of untoward reactions to iodinated contrast material. A review of known mechanisms, symptoms of toxicity and therapy is presented for use of iodinated contrast media in patients. Toxic reactions are compared with the administration of a histamine releasing agent. Allergic and immunologic models are p...

Bouhassira, E.E.; Billett, H.H.; Fabry, M.E.; Hirsch, R.E.; Krishnamoorthy, R., 2016: The scientific legacy of Ronald L. Nagel (1936-2016), a true renaissance man. American Journal of Hematology 91(9): 865-866