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Identification of SNARE and cell trafficking regulatory proteins in the salivary glands of the lone star tick, Amblyomma americanum (L.)

, : Identification of SNARE and cell trafficking regulatory proteins in the salivary glands of the lone star tick, Amblyomma americanum (L.). Insect Biochemistry and Molecular Biology 32(12): 1711-1721

Prostaglandin E2 (PGE2) stimulates secretion of tick salivary gland proteins via a phosphoinositide signaling pathway and mobilization of intracellular Ca2+ (Qian et al., 1998; Yuan et al., 2000). Highly conserved intracellular SNARE (soluble NSF attachment protein receptors) complex proteins are associated with the mechanism of protein secretion in vertebrate and invertebrate neuronal and non-neuronal cells. Proteins in the salivary glands of partially fed female lone star ticks cross-react individually with antibodies to synaptobrevin-2 (vesicle (v)-SNARE), syntaxin-1A, syntaxin-2 and SNAP-25 (target (t)-SNAREs), cytosolic [alpha]/[beta] SNAP and NSF (N-ethylmaleimide-sensitive fusion protein), Ca2+ sensitive synaptotagmin, vesicle associated synaptophysin, and regulatory cell trafficking GTPases Rab3A and nSec1. V-SNARE and t-SNARE proteins form an SDS-resistant, boiling sensitive core complex in the salivary glands. Antibodies to SNARE complex proteins inhibit PGE2-stimulated secretion of anticoagulant protein in permeabilized tick salivary glands. We conclude that SNARE and cell trafficking regulatory proteins are present and functioning in the process of PGE2-stimulated Ca2+ regulated protein secretion in tick salivary glands.


PMID: 12429123

DOI: 10.1016/s0965-1748(02)00111-x

Other references

Bronstein, L.H.; Goldwater, L.J.; Kresky, B., 1953: Occupational potentialities of the older cardiac patient. Geriatrics 8(5): 252-252

Maseuov, T.M., 1963: On the ecological morphology of the lings of rodents. Izvestiya Akademii Nauk Kazakhskoi SSR Biol, 3: 42-54

Fava, G.; Invernizzi, A.; Sichirollo, A.E.; Verrengia, F.; Zonca, G.C., 1972: Technical bases of mammography. Minerva Medica 63(73): 3977-3983

Mayock, P., 1978: Hyperkeratosis of the nipples. Archives of Dermatology 114(8): 1245-1245

Lindenmann, J., 1973: Speculations on idiotypes and homobodies. Annales D'immunologie 124(2): 171-184

Pollack, J.B.; Ragent, B.; Boese, R.; Tomasko, M.G.; Blamont, J.; Knollenberg, R.G.; Esposito, L.W.; Stewart, A.I.; Travis, L., 1979: Nature of the ultraviolet absorber in the venus clouds: inferences based on pioneer venus data. Several photometric measurements of Venus made from the Pioneer Venus orbiter and probes indicate that solar near-ultraviolet radiation is being absorbed throughout much of the main cloud region, but little above the clouds or within the first one...

D.V.oomen, M.; Steendijk, P.; Lopes Cardozo, R.H.; Brouwer, H.A.A.; Van Bel, F.; Baan, J., 2000: Improved systolic function of the right ventricle in response to pulmonary hypertension in the newborn lamb. Pediatric Research 47(4 Part 2): 41A, April

Krupko, I.L.; Farshatov, M.N.; Glebov, I.I., 1966: Open (non-gunshot and gunshot) fractures of bones of the foot and their treatment. Ortopediia Travmatologiia i Protezirovanie 27(12): 8-12

Colville-Ebeling, B.; Lynnerup, N.; Banner, J., 2015: Fatal lawn mower related projectile injury. Fatal lawn mower related injuries are a relatively rare occurrence. In a forensic setting, the primary aim is to reconstruct the injury mechanism and establish the cause of death. A relatively rare, but characteristic type of injury is a so-called...

Yoshipa, D., 1967: Effects of nutrient deficiencies on the biosynthesis of nicotine In tobacco plants. Incorporation of precursors of the pyrrolidine ring such as L-glutamic acid-U-14C, DL-ornithine-2-14C, L-arginine-U-14C, agmatine-G-3H and putrescine-1.414C into nicotine was decreased by K, Ca, Mg, S and B deficiencies; incorporation of nicotinic...