+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Identification of Salmonella Enteritidis outer membrane proteins expressed during attachment to human intestinal epithelial cells

, : Identification of Salmonella Enteritidis outer membrane proteins expressed during attachment to human intestinal epithelial cells. Journal of applied microbiology2(1): 180-186

Aims: To express and identify the Salmonella enterica ser. Enteritidis outer membrane proteins that are involved in the attachment to human intestinal epithelial cells. Methods and Results: Salmonella Enteritidis attachment proteins were expressed by infection of human intestinal epithelial cells (int-407) with Salm. Enteritidis strain SE28, followed by pulse labelling with [35S]-methionine. Outer membrane proteins were separated by TritonX-114 phase partitioning, and were detected by Salm. Enteritidis-specific polyclonal antibody. Outer membrane proteins of molecular weights 82.3, 75.6, 49.3, 35.5 and 19.3 kDa were newly synthesized. Expression of 31.2 and 16 kDa proteins was up-regulated, whereas the expression of 40.7 kDa protein was down-regulated. Polyclonal antibodies against the 82.3 and 75.6 kDa proteins significantly (P < 0.05) reduced the binding of Salm. Enteritidis to int-407 cells in vitro. Conclusions: Outer membrane proteins 82.3 and 75.6 kDa are potentially involved in the attachment of Salm. Enteritidis to the intestinal mucosa. Significance and Impact of the Study: Outer membrane proteins 82.3 and 75.6 kDa identified in this study could be used as potential vaccines to block or reduce Salm. Enteritidis colonization in chickens.


PMID: 11849343

DOI: 10.1046/j.1365-2672.2002.01511.x

Other references

Solntseva, G.N., 2003: Structures, functions, and parameters of bulla tympanica in the dolphin Tursiops truncatus in a three-dimensional presentation. Doklady Biological Sciences 381: 551-554

Iatsozhinskiĭ, I.D.; Kulik, N.M.; Pen'kova, N.G.; Petrik, F.B., 1970: Blood coagulation factors in pulmonary hemorrhages and hemoptysis in pulmonary tuberculosis. Problemy Tuberkuleza 48(12): 37-40

Pizarro, F.; Olivares, M.; Maciero, E.; Krasnoff, G.; Cócaro, N.; Gaitan, D., 2016: Iron Absorption from Two Milk Formulas Fortified with Iron Sulfate Stabilized with Maltodextrin and Citric Acid. Fortification of milk formulas with iron is a strategy widely used, but the absorption of non-heme iron is low. The purpose of this study was to measure the bioavailability of two iron fortified milk formulas designed to cover toddlers´ nutrition...

Madrid-Miller, A.; Chávez-Sánchez, L.; Careaga-Reyna, G.; Borrayo-Sánchez, G.; Chávez-Rueda, K.; Montoya-Guerrero, S.Armando.; Abundes Velazco, A.; Ledesma-Velasco, M.; Legorreta-Haquet, Mía.Victoria.; Blanco-Favela, F., 2015: Clinical outcome in patients with acute coronary syndrome and outward remodeling is associated with a predominant inflammatory response. Bmc Research Notes 7: 669-669

Mergoni, M., 1952: Clinical and anatomicopathologic study of temporal arteritis. Pathologica 44(693-4): 73-91

Chikami, K., 1997: Bracket for correcting dentition, method for producing the same, and method for improving adhesivity of polycarbonate. Official Gazette of the United States Patent & Trademark Office Patents 1203(4): 2802, Oct 28

Mitchelmore Lorraine; Smith Neville, 1994: West Muiron discovery, WA-155-P; new life for an old prospect. The discovery of the West Muiron gas and oil fields in 1992/3 (now renamed the Macedon-Pyrenees fields) was the culmination of an intensive two-year period of regional geophysical and geological evaluation by the WA-155-P Joint Venture in the Carn...

Anonymous, 1982: Farm level investment, part 2, land and buildings. This part of the survey examines investments in land and farm buildings between 1975 and 1977 by 1976 French farmers belonging to the CEMAGREF farm correspondent network, and working on a total of 72 282 ha, average 90.8 ha AA (Agricultural Area i...

Simic, D.V.; Tucakov, M., 2004: Numbers and local movements of pygmy cormorants Phalacrocorax pygmeus wintering in Belgrade. There is little recent information on Pygmy Cormorants Phalacrocorax pygmeus from Serbia. However, the confluence of the Sava and Danube rivers is known locally as a wintering site of the species. The study area lies within the city of Belgrade (U...

Springer, D.; Dziura, J.; Tamborlane, W.V.; Steffen, A.T.; Ahern, J.H.; Vincent, M.; Weinzimer, S.A., 2006: Optimal control of type 1 diabetes mellitus in youth receiving intensive treatment. Objective To investigate the impact of factors that might interfere with optimal glycemic control in youth with type 1 diabetes mellitus (T1DM) in, the current era of intensive management, including the interplay of race/ethnicity and socioeconomi...