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In situ hybridisation for identification and differentiation of Mycoplasma hyopneumoniae, Mycoplasma hyosynoviae and Mycoplasma hyorhinis in formalin-fixed porcine tissue sections

, : In situ hybridisation for identification and differentiation of Mycoplasma hyopneumoniae, Mycoplasma hyosynoviae and Mycoplasma hyorhinis in formalin-fixed porcine tissue sections. APMIS, Acta Pathologica, Microbiologica et Immunologica Scandinavica 109(10): 656-664

Oligonucleotide probes targeting 16S ribosomal RNA were designed for species-specific identification of 3 porcine Mycoplasma - M. hyopneumoniae, M. hyorhinis and M. hyosynoviae - using a fluorescent in situ hybridization assay. The specificity of the probes was evaluated using pure cultures as well as porcine tissue sections with artificial presence of Mycoplasma, and the probes were found specific for the target organisms.


DOI: 10.1034/j.1600-0463.2001.d01-129.x

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