+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Inhibition of cancer cell growth by crude extract and the phenolics of Terminalia chebula retz. fruit

, : Inhibition of cancer cell growth by crude extract and the phenolics of Terminalia chebula retz. fruit. Journal of Ethnopharmacology 81(3): 327-336

A 70% methanol extract of Terminalia chebula fruit, was studied for its effects on growth in several malignant cell lines including a human (MCF-7) and mouse (S115) breast cancer cell line, a human osteosarcoma cell line (HOS-1), a human prostate cancer cell line (PC-3) and a non-tumorigenic, immortalized human prostate cell line (PNT1A) using assays for proliferation ([(3)H]-thymidine incorporation and coulter counting), cell viability (ATP determination) and cell death (flow cytometry and Hoechst DNA staining). In all cell lines studied, the extract decreased cell viability, inhibited cell proliferation, and induced cell death in a dose dependent manner. Flow cytometry and other analyses showed that some apoptosis was induced by the extract at lower concentrations, but at higher concentrations, necrosis was the major mechanism of cell death. ATP assay guided chromatographic fractionation of the extract yielded ellagic acid, 2,4-chebulyl-beta-D-glucopyranose (a new natural product), and chebulinic acid which were tested by ATP assay on HOS-1 cell line in comparison to three known antigrowth phenolics of Terminalia, gallic acid, ethyl gallate, luteolin, and tannic acid. Chebulinic acid (IC(50) = 53.2 microM +/- 0.16) > tannic acid (IC(50) = 59.0 microg/ml +/- 0.19) > and ellagic acid (IC(50) = 78.5 microM +/- 0.24), were the most growth inhibitory phenolics of T. chebula fruit in our study.


PMID: 12127233

DOI: 10.1016/s0378-8741(02)00099-5

Other references

McAllister-Lucas, L.M.; Haik, T.L.; Colbran, J.L.; Sonnenburg, W.K.; Seger, D.; Turko, I.V.; Beavo, J.A.; Francis, S.H.; Corbin, J.D., 1995: An essential aspartic acid at each of two allosteric cGMP-binding sites of a cGMP-specific phosphodiesterase. The amino acid sequences of all known cGMP-binding phosphodiesterases (PDEs) contain internally homologous repeats (a and b) that are 80-90 residues in length and are arranged in tandem within the putative cGMP-binding domains. In the bovine lung...

Conrath Verrier M.; Dietl M.; Tramu G., 1984: Cholecystokinin like immunoreactivity in the dorsal horn of the spinal cord of the rat a light microscopic and electron microscopic study. Cholecystokinin-like immunoreactivity was investigated with an indirect immunoperoxidase technique in the whole spinal cord with the light microscope, and in the dorsal horn with EM. Intraparenchymal injections of colchicine were performed to allo...

Houck, K.; Nikrui, N.; Duska, L.; Chang, Y.; Fuller, A.F.Jr.; Bell, D.; Goodman, A., 2000: Borderline tumors of the ovary: Correlation of frozen and permanent histopathologic diagnosis. Objective: To evaluate the correlation between the diagnosis of borderline tumor of the ovary by frozen and permanent pathology. Methods: All pathology reports with diagnoses of borderline tumor of the ovary between 1980 and 1998 at Massachusetts...

Quee, T.C., 1982: Spiramycin and the specific plaque hypothesis: a new concept in plaque control. Journal 48(2): 121-127

Gorpinchenko I.I., 1986: Endocrine function of the testes gonadotropic and lactotropic activity of the adenohypophysis in the impotence of elderly men. Problemy Endokria 32(2): 35-36

Casey, R.P.; Njus, D.; Radda, G.K.; Sehr, P.A., 1976: Atp evoked catecholamine release in chromaffin granules osmotic lysis as a consequence of proton translocation. Bovine adrenal chromaffin granules suspended in Cl--containing media release catecholamine and protein when ATP is added. This phenomenon is inhibited in hyperosmotic media and in the presence of uncouplers of oxidative phsophorylation. Release re...

Peterson, R.T., 2004: HMO middle manager perceptions of the legality of specified practices. If Health Maintenance Organizations (HMOs) are to survive and prosper, they must abide by a large volume of potentially disruptive laws and regulations at the federal, state, and local levels. Some have suffered extreme financial loss and even bee...

Ye, C., 2006: Low-loss tunable filter based on optical rotatory dispersion. The optical arrangement of a spectral filter without an intermediate polarizer that was developed based on optical rotatory dispersion and test measurement results are presented and described. The filter uses three dispersive polarization rotators...

Baker, J.T.; Bloch, D.A., 1994: A quantitative method for evaluating user accuracy of a qualitative physician office diagnostic test. Clinical Chemistry 40(6): 1000

Bortkiewicz, A.; Zmyslony, M.; Gadzicka, E.; Szymczak, W., 1996: Evaluation of some parameters of the circulatory system function in various occupational groups of workers exposed to high frequency electromagnetic fields: II. Electrocardiographic changes. The effect of electromagnetic fields (EMF) on the circulatory and nervous systems has been the subject of great interest for many years, since electric impulses generated in these systems by outer electric and magnetic fields can theoretically dis...