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Intestinal infection with Giardia spp. reduces epithelial barrier function in a myosin light chain kinase-dependent fashion


, : Intestinal infection with Giardia spp. reduces epithelial barrier function in a myosin light chain kinase-dependent fashion. Gastroenterology 123(4): 1179-1190

Giardiasis causes malabsorptive diarrhea, and symptoms can be present in the absence of any significant morphologic injury to the intestinal mucosa. The effects of giardiasis on epithelial permeability in vivo remain unknown, and the role of T cells and myosin light chain kinase (MLCK) in altered intestinal barrier function is unclear. This study was conducted to determine whether Giardia spp. alters intestinal permeability in vivo, to assess whether these abnormalities are dependent on T cells, and to assess the role of MLCK in altered epithelial barrier function. Immunocompetent and isogenic athymic mice were inoculated with axenic Giardia muris trophozoites or sterile vehicle (control), then assessed for trophozoite colonization and gastrointestinal permeability. Mechanistic studies using nontransformed human duodenal epithelial monolayers (SCBN) determined the effects of Giardia on myosin light chain (MLC) phosphorylation, transepithelial fluorescein isothiocyanate-dextran fluxes, cytoskeletal F-actin, tight junctional zonula occludens-1 (ZO-1), and MLCK. Giardia infection caused a significant increase in small intestinal, but not gastric or colonic, permeability that correlated with trophozoite colonization in both immunocompetent and athymic mice. In vitro, Giardia increased permeability and phosphorylation of MLC and reorganized F-actin and ZO-1. These alterations were abolished with an MLCK inhibitor. Disruption of small intestinal barrier function is T cell independent, disappears on parasite clearance, and correlates with reorganization of cytoskeletal F-actin and tight junctional ZO-1 in an MLCK-dependent fashion.

US$19.90

PMID: 12360480

DOI: 10.1053/gast.2002.36002


Other references

Rochester, M.; Armitage, J.; Sanders, M.; Christmas, P., 2012: Self Management Activation Randomised Trial for Prostatitis (SMART-P): study protocol for a randomised controlled trial. Chronic prostatitis otherwise known as chronic pelvic pain syndrome is a common urological diagnosis that causes many men significant morbidity and has a detrimental effect on their quality of life. Standard treatment with antibiotics and simple a...

Huh, J.W.; Yoo, J.; Kim, M.S.; Choi, K.H.; Jue, M.S.; Park, H.J., 2016: Late-onset bulky naevocytoma of the perineum masquerading as a malignant melanoma. NlmCategory="UNASSIGNED">Bulky naevocytoma of the perineum is a very rare variant of giant congenital melanocytic naevus (GCMN). It presents as a bulky naevocytic tumour in the perineal region with characteristic histological findings...

Hildebrandt P.; Birch K.; Sestoft L.; Volund A., 1983: Sub cutaneous absorption of insulin monotard. Acta Endocrinologica Supplementum 103(257): 17

Ratheesh Ramakrishnan, A S Rajawat, 2012: Simulation of suspended sediment transport initialized with satellite derived suspended sediment concentrations. Suspended sediment transport in the Gulf of Kachchh is simulated utilizing the suspended sediment concentration (SSC) derived from Oceansat OCM imagery, as the initial condition in MIKE-21 Mud Transport model. Optimization of the model mud paramet...

Geboes, K.; Geboes, K.P.; Ectors, N., 1993: The histological classification of Barretts esophagus. Acta Endoscopica 22(5): 485-496, 1992

Shiffer, J.D.; Sandweiss, L.; Bose, S., 2001: The "polka dot" cell. Acta Cytologica 45(5): 903-905

Gallico, E.; Mayr, G., 1950: Magnetic behavior of certain synthetic estrogens. Bollettino Della Societa Italiana di Biologia Sperimentale 26(3): 264-265

Kirpichnikov V.S., 1990: Genetic studies of pacific salmon. Zhurnal Obshchei Biologii 51(1): 15-35

Tandon, P.N., 1964: Brain Stem Hemorrhage In Cranio-Cerebral Trauma. Brain stem hemorrhage is quite common in cases of fatal head injuries, and was present in 49 (37%) out of a total of 132 cases examined post-mortem. The most common site of these hemorrhages is pons either alone or in association with mid-brain, t...

Lee, T-Yi.; Bretaña, N.Arvin.; Lu, C-Tsung., 2011: PlantPhos: using maximal dependence decomposition to identify plant phosphorylation sites with substrate site specificity. Protein phosphorylation catalyzed by kinases plays crucial regulatory roles in intracellular signal transduction. Due to the difficulty in performing high-throughput mass spectrometry-based experiment, there is a desire to predict phosphorylation...