+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Isolation of tobacco ubiquitin-conjugating enzyme cDNA in a yeast two-hybrid system with tobacco ERF3 as bait and its characterization of specific interaction

, : Isolation of tobacco ubiquitin-conjugating enzyme cDNA in a yeast two-hybrid system with tobacco ERF3 as bait and its characterization of specific interaction. Journal of Experimental Botany 54(385): 1175-1181

Tobacco ETHYLENE-RESPONSIVE FACTOR3 (ERF3) is a member of the ERF-domain transcription factors and has a transcriptional repressor activity, whereas other ERF proteins show activation activity. To understand the regulation of ERF3-repressor activity, protein(s) were screened which interact with ERF3 in a yeast two-hybrid system. A partial sequence (B8) of NtUBC2, a tobacco ubiquitin-conjugating enzyme was isolated. This B8 specifically interacted with ERF3 in the yeast two-hybrid system. Further analyses revealed that the region unique to ERF3 interacted with B8. The physiological functions of NtUBC2 and the stability of ERF3 are discussed in relation to the regulation of the repression activity of ERF3. Reprinted by permission of the publisher.


PMID: 12654868

DOI: 10.1093/jxb/erg136

Other references

Mulyukov, Z.; Pevzner, P.A., 2002: EULER-PCR: finishing experiments for repeat resolution. Genomic sequencing typically generates a large collection of unordered contigs or scaffolds. Contig ordering (also known as gap closure) is a non-trivial algorithmic and experimental problem since even relatively simple-to-assemble bacterial genom...

Sarno, A.; Caimi, G.; Amuso, M.; Adamo, M., 1973: CO 2 gradient in mitral valve diseases. Bollettino Della Societa Italiana di Cardiologia 18(11): 1075-1082

Strack, K.M.; Schulenberg, S.E., 2010: Understanding empowerment, meaning, and perceived coercion in individuals with serious mental illness. This study investigated the relationship among the variables of perceived coercion, psychiatric symptoms, empowerment, and meaning in an inpatient sample of individuals with serious mental illness (N=94). It was hypothesized that empowerment would...

Formaggio, F.; Peggion, C.; Toniolo, C.; Kaptein, B.; Broxterman, Q.B.; Ni, M.; Muzi, L.; Dal Pozzo, A., 2009: Synthesis and conformational studies of novel, side-chain protected, L-(alphaMe)Ser homo-peptides. Advances in Experimental Medicine and Biology 611: 63-64

Lilien, O.M., 1967: Cell growth and development. A model biologic system. Fertility and Sterility 18(3): 318-330

Peyrin-Biroulet, L., 2009: Magnetic resonance imaging for Crohn's disease: is this really the end of colonoscopy?. Inflammatory Bowel Diseases 16(7): 1263-1264

Sato, T.; Yokota, S-Ichi.; Okubo, T.; Usui, M.; Fujii, N.; Tamura, Y., 2014: Phylogenetic association of fluoroquinolone and cephalosporin resistance of D-O1-ST648 Escherichia coli carrying blaCMY-2 from faecal samples of dogs in Japan. This study aimed to investigate the genetic association between fluoroquinolone (FQ) and/or cephalosporin (CEP) resistance in Escherichia coli isolates from dogs, and the risk to human health. We characterized E. coli clinical isolates, derived fr...

Hughes, D.A.; Marchetti, M.; Colombo, G., 2005: Cost minimization of on-demand maintenance therapy with proton pump inhibitors in nonerosive gastroesophageal reflux disease. On-demand proton pump inhibitor maintenance therapy is one of the suggested strategies for patients with nonerosive gastroesophageal reflux disease who achieve symptom remission after 4 weeks of continuous treatment. This review evaluates the diff...

Ludovico, P.; Côrte-Real, M., 2017: Yeast on the corner of life and death decisions. Mechanisms of Ageing and Development 161(Pt B): 199-200

Qiao, E-wei.; Zheng, H-fei., 2007: Raman spectroscopic studies of n-pentadecane under high temperature. The present paper investigates the Raman spectrum of n-pentadecane in diamond anvil cell under a temperature up to 350 degrees C. The result shows that the pressure increases at elevated temperature, but the effect of pressure on the stretching vi...