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Rapid detection of bovine milk adulteration in caprine milk


, : Rapid detection of bovine milk adulteration in caprine milk. Taiwanese Journal of Agricultural Chemistry and Food Science 39(6): 405-414

An indirect ELISA and a sandwich ELISA were successfully developed in this study for the rapid, quantitative detection of bovine milk adulteration in caprine milk. Polyclonal antibodies were raised in goats against bovine caseins. The antibodies were recovered from crude antisera by ammonium sulfate precipitation, and partially purified by passing through a DEAE anion exchange column. Both assays were able to quantitatively detect 0.0625 to 100% (v/v) bovine milk adulteration in caprine milk.

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