+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Role of calcium in protein folding and function of Tva, the receptor of subgroup A avian sarcoma and leukosis virus

, : Role of calcium in protein folding and function of Tva, the receptor of subgroup A avian sarcoma and leukosis virus. Journal of Virology 75(5): 2051-2058

Tva is the cellular receptor for subgroup A avian sarcoma and leukosis virus (ASLV-A). The viral receptor function of Tva is determined by a 40-residue cysteine-rich motif called the LDL-A module. In this study, we expressed and purified the wild-type (wt) Tva LDL-A module as well as several mutants and examined their in vitro folding properties. We found that, as for other LDL-A modules, correct folding and structure of the Tva LDL-A module is Ca2+ dependent. When calcium was present during in vitro protein folding, the wt module was eluted as a single peak by reverse-phase high-pressure liquid chromatography. Furthermore, two-dimensional nuclear magnetic resonance (NMR) spectroscopy gave well-dispersed spectra in the presence of calcium. In contrast, the same protein folded in vitro in the absence of calcium was eluted as multiple broad peaks and gave a poorly dispersed NMR spectrum in the presence of calcium. The calcium affinity (Kd) of the Tva LDL-A module, determined by isothermal titration calorimetry, is approximately 40 microM. Characterization of several Tva mutants provided further evidence that calcium is important in protein folding and function of Tva. Mutations of the Ca2+-binding residues (D46A and E47A) completely abrogated the Ca2+-binding ability of Tva, and the proteins were not correctly folded. Interestingly, mutations of two non-calcium-binding residues (W48A and L34A) also exerted adverse effect on Ca2+-dependent folding, albeit to a much less extent. Our results provide new insights regarding the structure and function of Tva in ASLV-A entry.


PMID: 11160709

DOI: 10.1128/JVI.75.5.2051-2058.2001

Other references

Erol, N.; Topbas, S.; Sahin, A., 2007: Retinal break following an intravitreal triamcinolone acetonide injection. Purpose To report a case of an unusual localization of retinal break following intravitreal triamcinolone acetonide (IVTA) injection.Subjects and methods A 58-year-old male patient was admitted to our department for visual loss lasting more than...

MacEwan, D.W., 1995: Reducing imaging costs in Manitoba, Canada. Manitoba, Canada, has serious debt and deficit problems and spends one-third of its revenue on health care. Manitoba Health, the single government carrier, has requested a 5-year management plan from the Provincial Imaging Advisory Committee to ma...

Brandner R., 1982: Trassic tectonics and sedimentation of the Tethys and their importance for the formation of alpine deposits of lead and zinc. Geologische Vereinigung 72: 20-21

Etzrodt H.; Schleyer M.; Fehm H.L.; Voigt K.H.; Pfeiffer E.F., 1974: Radiometric assay for animal growth hormone. Acta Endocrinologica Supplementum 184:

Krastev, M.; Gaidarska, V.; Toteva, M., 2002: Effect of some factors on length of production life of cows of black and white breed. II. Age and live weight at first conception, respectively calving. Study was carried out with the aim to determine the effect of live weight at first insemination and the age at first calving on longevity and length of production life of cows, daughters of Holstein Friesian bulls. Data for live weight at first co...

Manabe, Y.; Araki, M.; Takeda, K.; Yokota, S.; Kimura, T., 1989: Pseudohypoparathyroidism with striopallidodentate calcification--a case report and review of the literature. One case of calcification in the striopallidodentate system associated with pseudohypoparathyroidism type I is described. Striopallidodentate calcification accompanying a disturbance in calcium metabolism is, to our knowledge, extremely rare with...

Buchwald, D.; Kaltschmidt, C.; Haardt, H.; Laczkovics, A.; Reber, D., 2008: Autologous platelet gel fails to show beneficial effects on wound healing after saphenectomy in CABG patients. Wound healing impairment in the leg after removal of the saphenous vein within the framework of a coronary artery bypass graft (CABG) operation represents a clinically significant problem. Patients suffer from this complication, and treatment of t...

Pillon, B.; Deweer, B.; Michon, A.; Malapani, C.; Agid, Y.; Dubois, B., 1994: Are explicit memory disorders of progressive supranuclear palsy related to damage to striatofrontal circuits? Comparison with Alzheimer's, Parkinson's, and Huntington's diseases. To test the hypothesis that memory disorders of subcortico-frontal dementia result mainly from inefficiency of retrieval processes of stored information, we compared verbal learning in 15 patients with progressive supranuclear palsy, prototypical...

Balogh, R.; Wood, J.; Dobranowski, K.; Lin, E.; Wilton, A.; Jaglal, S.B.; Gemmill, M.; Lunsky, Y., 2016: Low-trauma fractures and bone mineral density testing in adults with and without intellectual and developmental disabilities: a population study. NlmCategory="UNASSIGNED">Individuals with intellectual and developmental disabilities (IDD) are at risk for low-trauma fractures. We investigated the rate of low-trauma fractures and the odds of BMD testing in adults with/without IDD....

Posteraro, G., 1953: Value of auxometric study of the circumference of the cranium in infants. Athena; Rassegna Mensile di Biologia, Clinica E Terapia 19(10-11): 366-368