+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Ag-ELISA and PCR for monitoring the vaccination of cattle against Taenia saginata cysticercosis using an oncospheral adhesion protein (HP6) with surface and secreted localization

, : Ag-ELISA and PCR for monitoring the vaccination of cattle against Taenia saginata cysticercosis using an oncospheral adhesion protein (HP6) with surface and secreted localization. Tropical Animal Health and Production 37(2): 103-120

A Taenia saginata oncosphere-derived adhesion protein (HP6) with surface and secreted localization was used to successfully vaccinate calves against oral challenge with T. saginata eggs. In contrast, vaccination using a combination of T. saginata oncosphere-derived peptides, selected on the basis of their antigenic index, and including three derived from the HP6 molecule (HP6-1, HP6-2 and HP6-3), was unsuccessful. This either indicated that the wrong peptides were selected or, in the case of the HP6 protein, that the protective epitope is conformational in nature. The protection experiments were monitored using a parasite antigen detection ELISA (HP10 Ag-ELISA), which allowed the early determination of the success of the vaccination protocol, subsequently confirmed at autopsy. PCR assays were used for the first time to confirm the presence of T. saginata DNA in lesions recovered at autopsy and thus verify the parasite origin of the lesions.


PMID: 15742866

DOI: 10.1023/b:trop.0000048459.98067.99

Other references

Simon J., 1985: The effect of gradated nitrogen rates on the floristic composition and nutritive value of permanent grassland. Rostlinna Vyroba 31(4): 369-376

Rubchenkov, P.N., 1988: Assessing the viability of bacteria by their total dehydrogenase activity. Dehydrogenase activity was measured with the aid of 2,3,5-triphenyltetrazolium at 4, 18 and 37 degrees C in freeze-dried vaccine strains of Pasteurella, Salmonella and Listeria after storage for 1, 2 and 3 months. Activity of the enzyme was correl...

Hecker, R.; Colpan, M.; Riesner, D., 1985: High-performance liquid chromatography of DNA restriction fragments. High-performance liquid chromatography on Nucleogen-DEAE 4000-10 has been applied to several problems of the isolation of DNA restriction fragments. Large amounts of DNA fragments of high purity are necessary for biophysical studies and for molecu...

A.A.tia, H.M.; Sherif, A.M.; Hossain, M.M.shaddeque; Ahmed, Y.H., 1998: The demographic and clinical spectrum of Arab versus Asian patients with ankylosing spondylitis in the UAE. Ankylosing spondylitis is a rather uncommon condition in the UAE. Over a period of 10 years. 28 hospital-based patients diagnosed as having AS were retrospectively studied. They included 17 Arabs and 11 Asians. The onset of AS in most patients in...

Azad, N.; Gondal, M.; Abbas, N.; Shahid, A., 2015: Frequency of depression and anxiety in patients attending a diabetes clinic. Depression is very common among people with type-2 diabetes and is associated with poor treatment compliance and outcome. This study was done to see the frequency of depression in patients with established type-2 diabetes attending a diabetes clin...

Chen, J.; Wang, F.; Liu, Q.; Du, J., 2015: Antibacterial polymeric nanostructures for biomedical applications. The high incidence of bacterial infection and the growing resistance of bacteria to conventional antibiotics have resulted in the strong need for the development of new generation of antibiotics. Nano-sized particles have been considered as novel...

Pegg, S.E., 1955: Seasonal calving for dairy cows. Production of milk and butter fat as affected by month of calving is given for each month of the year, totaling the years 1948-1954, for all Queensland and for 8 separate districts. The average production of butterfat by quarters for cows from all...

Palk, M.L., 1962: In-service education: its application in the Victorian Order of Nurses. Canadian Nurse 58: 424-426

Apple, D.F., 1993: Re-engineering. Circulatory disturbances of the upper extremities may be affected by blockade of the ganglion stellatum. This blockade is possible by electrical stimulation with pocket stimulators. Frequent blockades of the ganglion stellatum are possible, simple...

Reed M.J.; Lewis T.L.; Maurer D.; Wyngaarden P.; Brent H.P., 1989: A comparison of three acuity card procedures. Investigative Ophthalmology & Visual Science 30(3 SUPPL): 143