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Association of increased type I collagen expression and relative stromal overgrowth in mouse epididymis neonatally exposed to diethylstilbestrol

, : Association of increased type I collagen expression and relative stromal overgrowth in mouse epididymis neonatally exposed to diethylstilbestrol. Molecular Reproduction and Development 72(3): 291-298

The aim of this study was to investigate the molecular changes that underlie morphological changes in the epididymis following neonatal exposure to potent synthetic estrogen, namely diethylstilbestrol (DES). Newborn male mice were subcutaneously injected with DES or endogenous estrogen, namely 17 beta -estradiol (E2) (5 micro g/mouse/day), for the first 5 days. At the age of 2, 4, and 8 weeks, epididymides of the mice were dissected. Characteristic morphological abnormality, such as relative stromal overgrowth, was observed at the age of 2 weeks in the epididymis of DES-treated mice, but not in E2-treated mice. Microarray and real-time RT-PCR analyses revealed that the expression levels of procollagen type I alpha 1 (col1a1) and col1a2 genes were markedly upregulated at the age of 2 weeks in the epididymis of DES-treated mice in comparison with the control. Western blot analysis revealed that type I collagen protein expression level in epididymis of DES-treated mice was elevated at the age of 2 weeks. In situ hybridization analysis revealed that the signals of col1a1 mRNA were detected similarly throughout the stromal tissue of epididymis at the age of 2 weeks in control and DES- and E2-treated mice. The gene expression level of epididymal type III collagen (col3a1), which is found in many stromal connective tissues as well as type I collagen, did not change at the age of 2 weeks in all groups. These results suggest that the increased type I collagen expression is associated with the relative stromal overgrowth in the epididymis of DES-treated mice.


PMID: 16086434

DOI: 10.1002/mrd.20347

Other references

Oberg, G.; Mellander, O., 1955: Antibody formation and nutrition. 2. Antibody titre in lambs and goats fed on homologous and heterologous milk using influenza and diphtheria vaccine as antigens. For part I see Abet. 3807, Vol. 23. 2. The experiment reported in the earlier paper was repeated with 5 more pairs of twin lambs, with similar results. Ten goat kids were removed from their dams when 2 days old and bottle-fed with fresh cow's...

Khalid, A.; Demetris, A.J.; Whitcomb, D.C.; Sasatomi, E.; Swalsky, P.; Finkelstein, S., 2002: Genetic abnormalities in an acinar cell tumor of the pancreas The role of EUS guided FNA cytology and microdissection based mutational genotyping. Gastroenterology 122(4 Suppl 1): A-120, April

Kane, M.A.; Napoli, J.L., 2010: Quantification of endogenous retinoids. Numerous physiological processes require retinoids, including development, nervous system function, immune responsiveness, proliferation, differentiation, and all aspects of reproduction. Reliable retinoid quantification requires suitable handling...

Levin, B.S.; Lominski, I., 1934: Influence de la lecithine colloidale sur le phenomene de la lyse microbienne par le bacteriophage. Colloidal lecithin, in dilutions as great as 1/2000, exerts an inhibitive action on Staphylococcus phage. The phage is not destroyed and can be recovered by dilution of the lecithin-phage mixture.

Chen, X.; Li, Z.; Hu, X.; Kusuoka, Y., 2010: Morphology, morphogenesis and gene sequence of a freshwater ciliate, Pseudourostyla cristata (Ciliophora, Urostyloidea) from the ancient Lake Biwa, Japan. The urostyloid freshwater ciliate Pseudourostyla cristata was recorded for the first time from Lake Biwa, a 4-million-year-old lake located in Shiga Prefecture, Japan. Its morphology and morphogenesis were investigated using live observation and p...

Zhang, N.; Miyamoto, N.; Tanaka, R.; Mochizuki, H.; Hattori, N.; Urabe, T., 2008: Activation of tyrosine hydroxylase prevents pneumonia in a rat chronic cerebral hypoperfusion model. Pneumonia is a common complication with the highest attributable proportion of deaths in patients with stroke. Cilostazol is a potent type III phosphodiesterase inhibitor, approved as an anti-platelet aggregation agent. The present study was desig...

Rhodin, J.A.; Janson, A.M., 1981: Intravital and electron microscopic analysis of the arterioles by immersion and perfusion technics of fixation of the rat mesentery. The technique of the intravital microscopic control, videotaping, cinemoregistration and per single-frame analysis made it possible to demonstrate for the first time that a double fixation in glutaraldehyde and osmium has no effect on the arteriol...

Apatu R.S.K.; Barnes R.J., 1986: The release of glucose from the liver of fetal neonatal and adult sheep in response to the infusion of catecholamines and glucagon in vivo. Journal of Physiology (Cambridge) 378: 86P

Metz, R.; Sohn, W., 2015: Projection of prisoner numbers. The past and future development of occupancy rates in prisons is of crucial importance for the judicial administration of every country. Basic factors for planning the required penal facilities are seasonal fluctuations, minimum, maximum and avera...

McCLUNG.L.S.; TOABE.R., 2014: The nonreliability of the benzidine blood reaction for the detection of Clostridium parabotulinum. Journal of Bacteriology 50: 715-715