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Continuous production of butanol by Clostridium acetobutylicum immobilized in a fibrous bed bioreactor

, : Continuous production of butanol by Clostridium acetobutylicum immobilized in a fibrous bed bioreactor. Applied Biochemistry and Biotechnology 115(1/3): 887-898

We explored the influence of dilution rate and pH in continuous cultures of Clostridium acetobutylicum. A 200-mL fibrous bed bioreactor was used to produce high cell density and butyrate concentrations at pH 5.4 and 35degreesC. By feeding glucose and butyrate as a cosubstrate, the fermentation was maintained in the solventogenesis phase, and the optimal butanol productivity of 4.6 g/(L h) and a yield of 0.42 g/g were obtained at a dilution rate of 0.9 h-1 and pH 4.3. Compared to the conventional acetone-butanol-ethanol fermentation, the new fermentation process greatly improved butanol yield, making butanol production from corn an attractive alternative to ethanol fermentation.


PMID: 15054240

DOI: 10.1385/abab:115:1-3:0887

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