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Effect of ethanol on catalytic activity of papain


, : Effect of ethanol on catalytic activity of papain. Journal of Tropical and Subtropical Botany 13(4): 329-332

The alterations of papain activity and conformation were studied under conditions of pretreatment with ethanol solutions at different concentrations, different pretreatment times, and for various reaction concentrations. The results showed that papain activity was increased and the Km value declined when treated with ethanol. Ultraviolet difference spectra showed that the secondary structure of papain altered in ethanol solutions.

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