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Isolation of an N-alkylated benzylamine derivative from Pseudomonas putida BTP1 as elicitor of induced systemic resistance in bean


, : Isolation of an N-alkylated benzylamine derivative from Pseudomonas putida BTP1 as elicitor of induced systemic resistance in bean. Molecular Plant-Microbe Interactions 18(6): 562-569

Root treatment of Phaseolus vulgaris with the nonpathogenic Pseudomonas putida BTP1 led to significant reduction of the disease caused by the pathogen Botrytis cinerea on leaves. The molecular determinant of P. putida BTP1 mainly responsible for the induced systemic resistance (ISR) was isolated from cell-free culture fluid after growth of the strain in the iron-poor casamino acid medium. Mass spectrometry analyses performed on both the bacterial product and synthetic analogues revealed a polyalkylated benzylamine structure, with the quaternary ammonium substituted by methyl, ethyl, and C13 aliphatic groups responsible for the relative hydrophobicity of the molecule. The specific involvement of the N-alkylated benzylamine derivative (NABD) in ISR elicitation was first evidenced by testing the purified compound that mimicked the protective effect afforded by crude supernatant samples. The evidence was supported by the loss of elicitor activity of mutants impaired in NABD biosynthesis. Our experiments also showed that other iron-regulated metabolites secreted by the strain are not involved in ISR stimulation. Thus, these results indicate a wider variety of Pseudomonas determinants for ISR than reported to date.

US$19.90

PMID: 15986926

DOI: 10.1094/MPMI-18-0562


Other references

Majori L.; Petronio F.; Nedoclan G.; Barbieri A., 1973: Marine pollution by hydro carbons in the northern adriatic sea. Revue Internationale d'Oceanographie Medicale 31-32: 137-169

Delgado, Jp, 1980: An old sailing ship yields a cache of champagne. Wines and vines 61(6): 52-53

Morpurgo, E., 1949: The nerve supply of the omasum of domestic ruminants. The course of the nerve fibres in the omasum of domestic ruminants was described. M. observed a nerve ganglion in the greater curvature of the omasum of an eight inch bovine fetus and discussed its function.

Hines, J.; Fluharty, S.J.; Yee, D.K., 2001: Chimeric AT1/AT2 receptors reveal functional similarities despite key amino acid dissimilarities in the domains mediating agonist-dependent activation. Chimeric AT1/AT2 angiotensin II (AngII) receptors in which the sixth and/or seventh transmembrane-spanning domains of the AT2 receptor were substituted into the AT1 receptor were used to investigate the activation mechanisms of the two receptor su...

Hensel, M.; Shea, J.E.; Waterman, S.R.; Mundy, R.; Nikolaus, T.; Banks, G.; Vazquez Torres, A.; Gleeson, C.; Fang, F.C.; Holden, D.W., 1998: Genes encoding putative effector proteins of the type III secretion system of Salmonella pathogenicity island 2 are required for bacterial virulence and proliferation in macrophages. The type III secretion system of Salmonella pathogenicity island 2 (SPI-2) is required for systemic infection of this pathogen in mice. Cloning and sequencing of a central region of SPI-2 revealed the presence of genes encoding putative chaperones...

Thorsen, G., 1959: The use of dextrans as infusion fluids. Surgery, Gynecology & Obstetrics 109(1): 43-52

Anonymous, 2003: Vaccination coverage levels among Alaska Native children aged 19-35 months--National Immunization Survey, United States, 2000-2001. In 2000, a total of 118,846 persons indicated that their race/ethnicity was Alaska Native (AN), either alone or in combination with one or more other racial/ethnic groups. AN groups comprise 19% of the population of Alaska and 0.4% of the total U....

Liu, J.; Wang, F.; Ma, Z.; Wang, X.; Wang, Y., 2011: Structural determination of three different series of compounds as Hsp90 inhibitors using 3D-QSAR modeling, molecular docking and molecular dynamics methods. Hsp90 is involved in correcting, folding, maturation and activation of a diverse array of client proteins; it has also been implicated in the treatment of cancer in recent years. In this work, comparative molecular field analysis (CoMFA), comparat...

Matsuyama, M.; Kojima, A.; Katoh, H.; Kontani, K.; Kawai, M., 1989: Establishment of a congenic nude strain of rats, BUF/Mna-rnu. Suppression of thymomagenesis in heterozygous rats. The establishment of the new congenic strain of rats by introducing the rat nude gene into the BUF/Mna strain through successive backcrossing is described, and the phenotypic effects on the nu mutation in this strain are summarised.

Morrison, R.M., 1979: 'Approved' social worker. Royal Society of Health Journal 99(5): 216-218