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Linkage map of protein-protein interactions of Porcine teschovirus

, : Linkage map of protein-protein interactions of Porcine teschovirus. Journal of General Virology 86(Pt 10): 2763-2768

A yeast two-hybrid study was conducted to catalogue the protein-protein interactions of the Porcine teschovirus non-structural proteins. Five homodimer, three reciprocal heterodimer and four unidirectional heterodimer interactions were observed. While several interactions are similar to those described in previous studies using enteroviruses, such as homo- and heterodimeric interactions of the 2B, 3CD and 3D proteins, several were not found previously. Among these is the binding of the leader protein L to the proteinases 3C and 3CD. Unlike the poliovirus 3C, the teschovirus 3C proteinase dimerizes and interacts with 2BC, 3CD and 3D. The strongest interactions were observed for L-3C, L-3CD and 3C-3CD.


PMID: 16186230

DOI: 10.1099/vir.0.81144-0

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