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Molecular cloning and sequence variation of UDP-glucose pyrophosphorylase cDNAs from potatoes sensitive and resistant to cold sweetening

, : Molecular cloning and sequence variation of UDP-glucose pyrophosphorylase cDNAs from potatoes sensitive and resistant to cold sweetening. Journal of Plant Physiology 161(8): 947-955

RT-PCR was used to isolate seven cDNAs encoding uridine diphosphate-glucose pyrophosphorylase (UGPase) from six potato cultivars that differed markedly in their ability to sweeten in cold storage (2-4 degrees C). These sequences were compared to two potato UGPase-cDNAs previously published. All cDNAs were highly conserved (97.6-99.9%) and coded for polypeptides with 477 amino acids. The cDNAs could be placed into two sequence classes depending on whether they contained a BamH1 site at nucleotide positions 1315-1320. The presence of the BamH1 site (substitution of a C for a T at bp position 1320) did not lead to a change of an amino acid in the mature protein. There were 27 nucleotide polymorphisms that co-segregated along with the BamH1 site, five of which led to an amino acid change (i.e., bp positions (5) Thr for Ala; (30) Glu for Asp; (82) Lys for Asn; (445) Lys for Glu; and (450) Val for Ile). All of the encoded polypeptides contained the five highly conserved lysine residues located at positions 263, 329, 367, 409 and 410 that have been demonstrated necessary for catalytic activity of UGPase. All polypeptides had putative glycosylation sites at amino acid positions 168 (NQS) and 307 (NLS). The Ser at position 420 provided a putative site for phosphorylation as well as a binding motif for 14-3-3 proteins.


PMID: 15384406

DOI: 10.1016/j.jplph.2004.04.006

Other references

Negussie, B., 1992: Ethiopian midwives during the last year. Jordemodern 105(6): 202-203

Széll, Z.; Sréter, T.; Varga, I., 2003: Ivermectin toxicosis in a chameleon (Chamaeleo senegalensis) infected with Foleyella furcata. Both male and female Foleyella furcata were found in the subcutaneous tissue and abdominal cavity of an adult male wild-caught Senegalese chameleon (Chamaeleo senegalensis). This nematode species is endemic to Madagascar but has never been recorde...

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Myers, JP., 1981: Cross-seasonal interactions in the evolution of sandpiper social systems. Behavioral Ecology and Sociobiology, 83: 195-202

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Venables, A.W., 1970: Balloon atrial septostomy in complete transposition of great arteries in infancy. British Heart Journal 32(1): 61-65

Drolet, B.A.; Baselga, E.; Gosain, A.K.; Levy, M.L.; Esterly, N.B., 1997: Preauricular skin defects. Background: The term aplasia cutis is used to describe congenital localized defects of the skin. This affliction is the end result of various in utero events. Aplasia cutis of the face, although rare, has been associated with numerous dysmorphic f...

Levitz, M.; Selinger, M., 1970: Plasma estriol levels in class D diabetes of pregnancy. American Journal of Obstetrics and Gynecology 108(1): 82-84