geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Two MerR homologues that affect copper induction of the Bacillus subtilis copZA operon


, : Two MerR homologues that affect copper induction of the Bacillus subtilis copZA operon. Microbiology 149(Pt 12): 3413-3421

Copper ions induce expression of the Bacillus subtilis copZA operon encoding a metallochaperone, CopZ, and a CPx-type ATPase efflux protein, CopA. The copZA promoter region contains an inverted repeat sequence similar to that recognized by the mercury-sensing MerR protein. To investigate the possible involvement of MerR homologues in copZA regulation, null mutations were engineered affecting each of four putative MerR-type regulators: yyaN, yraB, yfmP and yhdQ. Two of these genes affected copper regulation. Mutation of yhdQ (hereafter renamed cueR) dramatically reduced copper induction of copZA, and purified CueR bound with high affinity to the copZA promoter region. These results suggest that CueR is a direct regulator of copZA transcription that mediates copper induction. Surprisingly, a yfmP mutation also reduced copper induction of copZA. Sequence analysis suggested that yfmP was cotranscribed with yfmO, encoding a putative multidrug efflux protein. The yfmPO operon is autoregulated: a yfmP mutation derepressed the yfmP promoter and purified YfmP bound the yfmP promoter region, but not the copZA promoter region. Since the yfmP mutant strain was predicted to express elevated levels of the YfmO efflux pump, it was hypothesized that copper efflux might be responsible for the reduced copZA induction. Consistent with this model, in a yfmP yfmO double mutant copper induction of copZA was normal. The results demonstrate the direct regulation of the B. subtilis copper efflux system by CueR, and indirect regulation by a putative multidrug efflux system. Reprinted by permission of the publisher.

US$19.90

PMID: 14663075

DOI: 10.1099/mic.0.26225-0


Other references

Novoa, R.A.; Honda, K.S.; Campagnaro, E.; Gerstenblith, M.R., 2014: Hypertensive emergency, matlike telangiectasias, and calciphylaxis in POEMS syndrome. JAMA Dermatology 150(6): 667-669

Brady J.G., 1966: Processing problems associated with some typical ceramic clays and shales of Ontario. Canadian Ceramic Soc Jour

Fligor, S.C.; Love, K.M.; Collier, B.R.; Lollar, D.I.; Hamill, M.E.; Benson, A.D.; Bradburn, E.H., 2017: Parathyroid hormone as a marker for hypoperfusion in trauma: a prospective observational study. Hyperparathyroidism is common in critical illness. Intact parathyroid hormone has a half-life of 3 to 5 minutes due to rapid clearance by the liver, kidneys, and bone. In hemorrhagic shock, decreased clearance may occur, thus making parathyroid ho...

Iacoboni, M.; Rayman, J.; Zaidel, E., 1997: Does the previous trial affect lateralized lexical decision?. We investigated the effect of previous trial variables on performance in the current trial in a lexical decision task with unilateral presentation of one letter string or bilateral simultaneous presentation of two different letter strings, one cue...

Hilber, K.; Galler, S., 1998: Effects of myofibrillar bundle diameter on the unloaded shortening velocity of skinned muscle fibres. Using both slack tests and force clamp experiments, the velocity of unloaded shortening (Vu; Vu(st), slack test; Vu(fc), force clamp) was determined for maximally Ca2+-activated myofibrillar bundles. These were obtained by mechanically splitting s...

McCrea, C.E.; West, S.G.; Kris-Etherton, P.M.; Lambert, J.D.; Gaugler, T.L.; Teeter, D.L.; Sauder, K.A.; Gu, Y.; Glisan, S.L.; Skulas-Ray, A.C., 2016: Effects of culinary spices and psychological stress on postprandial lipemia and lipase activity: results of a randomized crossover study and in vitro experiments. Data suggest that culinary spices are a potent, low-calorie modality for improving physiological responses to high fat meals. In a pilot study (Nā€‰=ā€‰6 healthy adults), we showed that a meal containing a high antioxidant spice blend attenuated p...

Jessel, R.; Haertel, S.; Socaciu, C.; Tykhonova, S.; Diehl, H.A., 2002: Kinetics of apoptotic markers in exogeneously induced apoptosis of EL4 cells. We investigated the time-dependence of apoptotic events in EL4 cells by monitoring plasma membrane changes in correlation to DNA fragmentation and cell shrinkage. We applied three apoptosis inducers (staurosporine, tubericidine and X-rays) and we...

Tikhonov K.B.; Yakovleva L.A., 1980: Effect of various methods of irradiation on the coronary vessels in an experiment. The effect of single and fractionated irradiation of the heart region with a cumulative dose of 2400 rad on the rabbit coronary vessels was studied by microangiography. Single and fractionated irradiation of the heart region induced persistent dis...

Deal, C.L.; Eberle, R.W.; Mazess, R.B.; Barden, H., 1992: Bone loss in patients treated with high dose corticosteroids. Arthritis & Rheumatism 35(9 SUPPL ): S315

Blackman; Brochard, 2000: Polydispersity exponent in homogeneous droplet growth. The homogeneous growth of D-dimensional hyperspherical droplets on a d-dimensional surface produces a bimodal distribution in the sizes of the droplets, with a roughly monodispersed distribution of larger droplets superimposed on a highly polydisp...