geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Adventitious shoot regeneration and rooting of Prunus serotina in vitro cultures


, : Adventitious shoot regeneration and rooting of Prunus serotina in vitro cultures. HortScience: a ication of the American Society for Horticultural Science 41(1): 193-201

A complete regeneration protocol was developed for Prunus serotina Ehrh., an important hardwood species for timber and sawlog production in the central and eastern United States. Nodal sections were cultured on Murashige and Skoog (MS) medium supplemented with 4.44 micromolar 6-benzylaminopurine (BA), 0.49 micromolar indole-3-butyric acid (IBA), and 0.29 micromolar gibberellic acid (GA3). In vitro leaf explants of three genotypes were placed on woody plant medium (WPM) supplemented with 0, 2.27, 4.54, or 6.81 micromolar thidiazuron (TDZ) in combination with 0, 0.54, 1.07, or 5.37 micromolar naphthaleneacetic acid (NAA), and on WPM supplemented with 0, 4.44, 8.88, or 13.32 micromolar BA in combination with 0, 0.54, 1.07, or 5.37 micromolar NAA. Cultures were maintained either in continuous darkness for 5 weeks, or in the dark for 3 weeks and then transferred to a 16-hour photoperiod. TDZ and the genotype had a significant effect on the number of shoots regenerated. The maximum mean number of shoots regenerated per explant (5.05 +/- 1.14) was obtained with 2.27 micromolar TDZ plus 0.54 micromolar NAA with the 3-week dark period then light treatment. The highest percent shoot regeneration (38.3) and mean number of shoots (4.13 +/- 0.97) was obtained with 6.81 micromolar TDZ plus 1.07 micromolar NAA. The highest rooting (27%) of adventitious shoots and number of roots per shoot (2.3 +/- 0.2) was obtained with 2.5 micromolar IBA when shoots were maintained for 7 days in the dark on rooting medium before transfer to a 16-hour photoperiod. The highest rooting (70%) of nodal explant-derived stock cultures and number of roots per shoot (2.7 +/- 0.9) was also obtained with 2.5 micromolar IBA, but when shoots were maintained for 4 days in the dark before transfer to a 16-hour photoperiod. In total, 86% of the plantlets survived acclimatization to the greenhouse and 100% survival after overwintering in cold-storage.

US$29.90


Other references

Arora, S.; Gonzales, I.M.; Hagelstrom, R.Tanner.; Beaudry, C.; Choudhary, A.; Sima, C.; Tibes, R.; Mousses, S.; Azorsa, D.O., 2010: RNAi phenotype profiling of kinases identifies potential therapeutic targets in Ewing's sarcoma. Ewing's sarcomas are aggressive musculoskeletal tumors occurring most frequently in the long and flat bones as a solitary lesion mostly during the teen-age years of life. With current treatments, significant number of patients relapse and sur...

Vetemaa, M.; Kalda, R.; Tambets, M., 2008: Success of embryonic development of reciprocal hybrids of bream Abramis brama L and white bream Blicca bjoerkna L. Success of embryonic development of reciprocal hybrids of bream Abramis brama and white bream Blicca bjoerkna was tested under laboratory conditions. Fertilization and embryonic development success of hybrids until hatching was high and comparabl...

Howard, S.K.; Gaba, D.M., 2006: Factors influencing vigilance and performance of anesthetists. As a group, anesthetists have been the leaders in medicine in the study of vigilance, performance, and safety. This review updates the work that has been done in the last year regarding the study of anesthetist vigilance and performance. Much of t...

Tsuruta J.; Yamamoto T.; Kozono K.; Kambara T., 1985: Application of a new method of antibody enzyme conjugation with maleimide derivative for immunohistochemistry hepatocellular production interstitial tissue distribution and renal cell reabsorption of plasma albumin in guinea pig. A new sophisticated method for enzyme-antibody conjugation developed in quantitative solid-phase enzyme immunoassay was an applicable method for immunohistochemistry; 1 that offered several advantages over present methods. Using a new maleimide de...

Juris Heipke, S.; Fuchs, R.; Motz, M.; Preac Mursic, V.; Schwab, E.; Soutschek, E.; Will, G.; Wilske, B., 1993: Genetic heterogenity of the genes coding for the outer surface protein C (OspC) and the flagellin of Borrelia burgdorferi. The ospC gene coding for the outer surface protein OspC and the fla gene coding for the flagellin have been investigated in three different Borrelia burgdorferi sensu lato strains. These strains (the North American strain B31 and the European stra...

Viret, O., 1996: Control strategies against grapevine diseases. An outline is given of the modes of action of fungicides used in viticulture. Integrated control schedules suitable for use in W. and S. Switzerland are presented for diseases caused by Phomopsis viticola, Pseudopeziza tracheiphila, Plasmopara vit...

Lagutina, N.Ia.; Saltykow, B.B.; Rozanova, N.S.; Vlasova, A.G.; Faĭnshteĭn, F.E., 1976: Immunohistochemical study of vessels in hypoplastic anemia. Sovetskaia Meditsina: 42-44

Malanda, M.; Dumoulin, M.; Clamagirand, V.; Boivin, P., 1999: Mycotoxins and toxinogenic potential of moulds on malting barley. Journal of the Institute of Brewing 105(3): 145, May-June

Marshall R.J.; Gee R.; Israel M.; Neave D.; Edwards F.; Dumble J.; Wong S.; Chan C.; Patel R.; E.A., 1990: The use of alternative therapies by auckland new zealand general practitioners. A survey of Auckland (New Zealand) general practitioners was undertaken to explore their use of, and attitudes to, alternative medicines. Three hundred and seventy randomly selected doctors, from a total of about 700 doctors practising in Auckland...

Lanham, William E.Jr, 2003: Dough feeding unit. A metering unit for dough and related foodstuffs having a dough feeder unit, support structure, dough hopper, effluent housing, drive motor and centerless helix, with the effluent housing including an inlet, center and outlet section. The centerle...