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Characterization of putative ligands for a fish gonadal androgen receptor in a pulp mill effluent


, : Characterization of putative ligands for a fish gonadal androgen receptor in a pulp mill effluent. Environmental Toxicology and Chemistry ruary; 25(2): 419-427

Fish exposed to pulp and paper mill effluents often become masculinized. A plausible hypothesis is that this is caused by activation of androgen receptors. The present study aimed to investigate if ligands for the fish gonadal androgen receptor (AR2) are present in pulp mill effluent and to characterize/identify these compounds. Extracts of both primary and biologically treated effluents from a Swedish kraft pulp mill were fractionated chemically. Fractions were tested in competitive binding assays for AR2 from ovaries of the Atlantic croaker (Micropogonias undulatus). Primary effluent contained 96 ng dihydrotestosterone equivalents/L, whereas biologically treated effluent was 16 times less potent. Further fractionations and assays of binding activities were performed on the primary effluent. Eight final fractions displaced androgen in the binding assay, and gas chromatography/mass spectrometry (GC/MS) analyses revealed that these contained 37 detectable compounds that were not present in inactive fractions. The majority were moderately polar compounds between 200 and 400 g/mol with hydroxyl/carbonyl groups. Two compounds were ruled out because of their lack of binding to AR2. The mass spectra of a third compound matched that of 4-hydroxy-3 (2-(4-hydroxy-3-methoxophenyl)ethyl)-5-metoxyacetophenon, but the remaining candidates could not be fully identified. A search for 21 known steroidal AR2 ligands showed that progesterone, a relatively strong AR2 ligand, was present in the primary effluent (1.6 [mu]g/L) but was removed during the biological treatment step. The detection of multiple fractions with significant binding activity indicates that a variety of compounds in effluents have the potential to masculinize fish near pulp mills via an androgen receptor-mediated mechanism.

US$19.90

PMID: 16519302

DOI: 10.1897/05-177r.1


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Köhler, C., 2016: Granulovacuolar degeneration: a neurodegenerative change that accompanies tau pathology. Granule-containing vacuoles in the cytoplasm of hippocampal neurons are a neuropathological feature of Alzheimer's disease. Granulovacuolar degeneration (GVD) is not disease-specific and can be observed in other neurodegenerative disorders an...

Lee, S.H.; Kwon, H.S.; Wang, Y.T.; Sheng, M., 2001: Subunit specific regulation of AMPA receptor endocytosis in hippocampal neurons. Internalization of AMPA receptors from synaptic membranes causes a depression of excitatory synaptic transmission, which occurs via a dynamin-dependent clathrin-mediated endocytic pathway. A variety of factors including AMPA, NMDA, and insulin hav...

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Heidrich, H.J.; Lupke, H., 1965: Examination of milk and blood of cows after oral and intravenous administration of Otrhomin thiocyanate preparation. In tests lasting 8 days, Otrhomin was administered (i) in 20-g. doses orally as an aqueous suspension to 6 healthy cows and (ii) in 30-ml. doses intravenously to 7 healthy cows after the evening milking. The milk thiocyanate levels for (i) decline...

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Scheel, E., 1983: Formaldehyde gas from laminated wood boards in the air of dwellings. Effect of the gas on children with bronchial asthma. Tidsskrift for den Norske Laegeforening 103(4): 283-286

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