+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Expression of KiSS-1, a metastasis suppressor gene, in trophoblast giant cells of the rat placenta

, : Expression of KiSS-1, a metastasis suppressor gene, in trophoblast giant cells of the rat placenta. Biochimica et Biophysica Acta 1678(2-3): 102-110

Metastin is encoded by a putative human metastasis suppressor gene KiSS-1, and is the cognate ligand of a G-protein-coupled receptor designated OT7T175. To study the physiological function(s) of metastin, we cloned rat and mouse KiSS-1 cDNAs both encoding 130-amino acid KiSS-1 proteins. Sequence analysis suggested that processing of the rat and mouse KiSS-1 proteins produces 52-amino-acid peptides, each with an amidated carboxyl terminal and with a single possible disulfide bond, corresponding to rat and mouse metastins. The carboxyl-terminal sequence of metastin, known to be essential for functional receptor interaction, was found to be highly conserved among humans and rodents. Real-time PCR analysis indicated that rat KiSS-1 mRNA showed the highest expression level in the cecum and colon. Since KiSS-1 mRNA and metastin are known to be abundant in human placenta, we further studied the localization of KiSS-1 and OT7T175 mRNAs in rat placenta by in situ hybridization. KiSS-1 and OT7T175 mRNAs were specifically detected in trophoblast giant cells at embryonic day 12.5, and the transcripts in the cells gradually decreased during placental maturation. These results suggest that metastin/OT7T175 signaling may participate in implantation of the mammalian embryo, placenta formation, and maintenance of pregnancy.


PMID: 15157736

DOI: 10.1016/j.bbaexp.2004.02.005

Other references

Bystryn, J-Claude.; Reynolds, S.R., 2005: Melanoma vaccines: what we know so far. Vaccines are a promising but still experimental treatment for melanoma. They are intended to stimulate immune responses against melanoma and by so doing, increase resistance against and slow the progression of this cancer. Key requirements for vac...

Uryvaev, L.V.; Sokolova, T.M.; Ershov, F.I.; Zhdanov, V.M., 1972: Study of the phenomenon of viral RNA complexing with cellular proteins. Voprosy Virusologii 17(6): 670-676

Rengei, B.; Mindszenty, L.; Ember, M.; Csiszár, B., 1973: The effect of a single oral ethanol dose on the cholinesterase, GOT, GPT, alkaline phosphatase activity and on the A vitamin level of human blood with special emphasis on poisonings with organic phosphoric acid insecticides. Zeitschrift für Rechtsmedizin. Journal of Legal Medicine 73(1): 45-51

Ohsawa, T.; Ide, Y., 2008: Global patterns of genetic variation in plant species along vertical and horizontal gradients on mountains. Aim To understand global patterns of genetic variation in plant species on mountains and to consider the significance of mountains for the genetic structure and evolution of plant species.Location Global.Methods We review published studies.Resutls...

Livingston, C.W.; Jr.; Moore, R.W.; Redmond, H.E.; Hardy, W.T., 1965: Polyarthritis virus-a cause of stiff lambs in Texas. The symptoms of "stiff lamb disease", observed among two to five-month-old lambs in Texas for at least 15 years, are described. Serological evidence of polyarthritis infection was obtained in six flocks by subjecting sera from affected l...

Stanley, S.W.U.; Haywood, R.W., 1987: Computer simulation of haulage tractor tests. A computer model is outlined, using various tractor parameters and traction mechanics to simulate p.t.o. dynamometer tests for engine performance and a road haulage test monitoring speed, load and fuel consumption for tractors over a known route....

Zazulia, A.R.; Videen, T.O.; Powers, W.J., 2009: Transient focal increase in perihematomal glucose metabolism after acute human intracerebral hemorrhage. Progressive perihematomal cell death over 3 to 4 days has been described after experimental intracerebral hemorrhage (ICH). We investigated whether progressive perihematomal damage occurs in human subjects by measuring relative changes in regional...

Chen, Y.; Wang, J., 2014: A membrane-based near-infrared fluorescence assay for detecting DNA methylation and transcription. DNA methylation is a common epigenetic modification. The epigenetic silencing of a variety of genes by hypermethylation of promoter-associated CpG islands is often associated with diseases. Therefore, a reliable but uncomplicated method for detect...

Yu, L.; Pedrini, G.; Osten, W.; Kim, M.K., 2007: Three-dimensional angle measurement based on propagation vector analysis of digital holography. We propose what we believe to be a novel method for highly accurate three-dimensional (3D) angle measurement based on propagation vector analysis of digital holography. Three-dimensional rotations in space can be achieved by use of a CCD camera an...

Tucker, JK., 1977: The identity of Conus columba Hwass. Hawaiian Shell News, 256: 10