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Quantification of mRNA encoding cytokines and chemokines in nasal biopsies from dogs with sino-nasal aspergillosis

, : Quantification of mRNA encoding cytokines and chemokines in nasal biopsies from dogs with sino-nasal aspergillosis. Veterinary Microbiology 114(3-4): 318-326

Canine sino-nasal aspergillosis is usually caused by Aspergillus fumigatus and is similar to human chronic erosive non-invasive fungal sinusitis. The pathogenesis of the disease is poorly understood. We investigated the nature of the local immune response mounted in canine sino-nasal aspergillosis. Quantitative RT-PCR was carried out on RNA isolated from nasal biopsies from diseased and control dogs, using specific assays designed to amplify mRNA encoding a panel of cytokines and chemokines. Canine sino-nasal aspergillosis was associated with significantly increased expression of mRNA encoding MCP-1, -2, -3 and -4, IL-8, IL-10, IL-18 and TNF-(Sa(B relative to controls (P < 0.01) but there was no difference between groups with respect to IL-4, IL-5, IL-6, IL-12, TGF-(Sb(B, and eotaxin-2 and -3. The up-regulation of proinflammatory cytokines and chemokines related to the influx of phagocytic cells might account for the localisation of this infection to the upper respiratory tract. The up-regulation of the expression of the immunomodulatory cytokine IL-10 in nasal tissue from affected dogs might be important in limiting the extent of local tissue destruction, but might also account for the fact that infected dogs are generally unable to clear this infection spontaneously.


PMID: 16387453

DOI: 10.1016/j.vetmic.2005.11.065

Other references

Scott, H.S.; Litjens, T.; Hopwood, J.J.; Morris, C.P., 1992: A common mutation for mucopolysaccharidosis type I associated with a severe Hurler syndrome phenotype. Mucopolysaccharidosis type I (MPS-I) is an autosomal recessive genetic disease caused by a deficiency of the glycosidase alpha-L-iduronidase which is required for the lysosomal degradation of the glycosaminoglycans heparan sulfate and dermatan sul...

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Liu, C.; Bai, R.; San Ly, Q., 2007: Selective removal of copper and lead ions by diethylenetriamine-functionalized adsorbent: behaviors and mechanisms. The selective removal of copper and lead ions from aqueous solutions by diethylenetriamine (DETA)-functionalized polymeric adsorbent was investigated. The adsorbent was prepared by amination of the micro-beads synthesized from glycidyl methacrylat...

Killander, A.; Schilling, R.F., 1963: Consecutive daily tests of vitamin B12 absorption by the urine radioactivity technique. PROCEEDINGS CONGR EUROPE SOC HAEMATOL 9(2 Pt 2): 1483-1494

Fitzgerald, M.; Einstein, A., 2001: Einstein: brain and behavior. Journal of Autism and Developmental Disorders 30(6): 620-621

Diaz, N.R.vera, J., 1989: Tentative identification of volatile components of pineapple juice from varieties Red Spanish and PR 1-67. Journal of agriculture of the University of Puerto Rico 73(3): 189-194

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