geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Quantification of mRNA encoding cytokines and chemokines in nasal biopsies from dogs with sino-nasal aspergillosis


, : Quantification of mRNA encoding cytokines and chemokines in nasal biopsies from dogs with sino-nasal aspergillosis. Veterinary Microbiology 114(3-4): 318-326

Canine sino-nasal aspergillosis is usually caused by Aspergillus fumigatus and is similar to human chronic erosive non-invasive fungal sinusitis. The pathogenesis of the disease is poorly understood. We investigated the nature of the local immune response mounted in canine sino-nasal aspergillosis. Quantitative RT-PCR was carried out on RNA isolated from nasal biopsies from diseased and control dogs, using specific assays designed to amplify mRNA encoding a panel of cytokines and chemokines. Canine sino-nasal aspergillosis was associated with significantly increased expression of mRNA encoding MCP-1, -2, -3 and -4, IL-8, IL-10, IL-18 and TNF-(Sa(B relative to controls (P < 0.01) but there was no difference between groups with respect to IL-4, IL-5, IL-6, IL-12, TGF-(Sb(B, and eotaxin-2 and -3. The up-regulation of proinflammatory cytokines and chemokines related to the influx of phagocytic cells might account for the localisation of this infection to the upper respiratory tract. The up-regulation of the expression of the immunomodulatory cytokine IL-10 in nasal tissue from affected dogs might be important in limiting the extent of local tissue destruction, but might also account for the fact that infected dogs are generally unable to clear this infection spontaneously.

US$19.90

PMID: 16387453

DOI: 10.1016/j.vetmic.2005.11.065


Other references

Scott, H.S.; Litjens, T.; Hopwood, J.J.; Morris, C.P., 1992: A common mutation for mucopolysaccharidosis type I associated with a severe Hurler syndrome phenotype. Mucopolysaccharidosis type I (MPS-I) is an autosomal recessive genetic disease caused by a deficiency of the glycosidase alpha-L-iduronidase which is required for the lysosomal degradation of the glycosaminoglycans heparan sulfate and dermatan sul...

Lin, C-King.; Chen, L-Ping.; Chang, H-Lin.; Sung, Y-Chuan., 2015: Underestimation of the coexistence of iron deficiencies and thalassemia minors: a single institution experience in Taiwan. Some physicians neglect the possible coexistence of an iron deficiency with a thalassemia minor and do not treat the iron deficiency accordingly. This motivated us to conduct this study. We retrospectively reviewed the records of 3892 patients who...

Schledermann, D.Ejersbo.; Vad, H., 2005: Boerhaave's syndrome--a life-threatening condition which is often diagnosed too late. Ugeskrift for Laeger 166(49): 4486-4488

Liu, C.; Bai, R.; San Ly, Q., 2007: Selective removal of copper and lead ions by diethylenetriamine-functionalized adsorbent: behaviors and mechanisms. The selective removal of copper and lead ions from aqueous solutions by diethylenetriamine (DETA)-functionalized polymeric adsorbent was investigated. The adsorbent was prepared by amination of the micro-beads synthesized from glycidyl methacrylat...

Killander, A.; Schilling, R.F., 1963: Consecutive daily tests of vitamin B12 absorption by the urine radioactivity technique. PROCEEDINGS CONGR EUROPE SOC HAEMATOL 9(2 Pt 2): 1483-1494

Fitzgerald, M.; Einstein, A., 2001: Einstein: brain and behavior. Journal of Autism and Developmental Disorders 30(6): 620-621

Diaz, N.R.vera, J., 1989: Tentative identification of volatile components of pineapple juice from varieties Red Spanish and PR 1-67. Journal of agriculture of the University of Puerto Rico 73(3): 189-194

Nazarov, V.U.; Vignale, G.; Chang, Y-C., 2009: Exact dynamical exchange-correlation kernel of a weakly inhomogeneous electron gas. The dynamical exchange-correlation kernel f{xc} of a nonuniform electron gas is an essential input for the time-dependent density-functional theory of electronic systems. The long-wavelength behavior of this kernel is known to be of the form f{xc}...

Yang, Z.; Xiong, S.J., 2009: Theoretical study of Y(n)O(2) and Y(n)O(2)(-) (n = 1-8) clusters. The structural, electronic, and magnetic properties of Y(n)O(2) and Y(n)O(2)(-) clusters, up to n = 8, have been systematically investigated by using the density-functional approach. Our theoretical results show that the geometries of the ground-s...

Lankston, Robert W., 2008: Effect of non-hydrostatic pore pressure on the depth to the base of the hydrate stability zone. The pressure term in the hydrate stability equation can be converted to depth to facilitate estimating the vertical extent of the hydrate stability zone (HSZ). The conversion from pressure to depth is generally made assuming that hydrostatic condi...