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Reagentless biosensor for phenolic compounds based on tyrosinase entrapped within gelatine film

, : Reagentless biosensor for phenolic compounds based on tyrosinase entrapped within gelatine film. Analytical and Bioanalytical Chemistry 383(7-8): 1127-1132

A simple and new reagentless phenolic compound biosensor was constructed with tyrosinase immobilized in the gelatine matrix cross-linked with formaldehyde. The morphologies of gelatine and gelatine/tryosinase were characterized by SEM. The tyrosinase retains its bioactivity when being immobilized by the gelatine film. Phenolic compounds were determined by the direct reduction of biocatalytically liberated quinone at -0.1 V vs SCE.


PMID: 16237545

DOI: 10.1007/s00216-005-0115-5

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